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160201s2016 xxu| s |||| 0|eng d |
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|a 9781493930647
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| 024 |
7 |
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|a 10.1007/978-1-4939-3064-7
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Receptor and Ion Channel Detection in the Brain :
|b Methods and Protocols /
|c edited by Rafael Luján, Francisco Ciruela.
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| 250 |
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|a 1st ed. 2016.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2016.
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| 300 |
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|a XVIII, 475 p. 84 illus., 57 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Neuromethods,
|v 110
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| 505 |
0 |
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|a Preface -- Production of high-quality antibodies for the study of receptors and ion channels in brain -- Co-immunoprecipitation from brain -- Sub-synaptic membrane fractionation -- Investigation of neurotransmitter receptors in brain slices using cell surface biotinylation -- Single nanoparticle tracking of surface ion channels and receptors in brain cells -- Radioligand binding detection of receptors in brain membranes -- Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain -- Fluorescent ligands and TR-FRET to study receptor-receptor interactions in the brain -- In situ Proximity Ligation Assay to study and understand the distribution and balance of GPCR homo- and heteroreceptor complexes in the brain -- Fluorescent in situ hybridization for sensitive and specific labeling -- Autoradiographic visualization of G protein-coupled receptors in brain -- Analysis of the expression profile and regional distribution of neurotransmitter receptors and ion channels in the central nervous system using histoblots -- Immunohistochemistry for ion channels and their interacting molecules: Tips for improving antibody accessibility -- Localization of GFP-tagged proteins at the electron microscope -- Pre-embedding methods in the localization of receptors and ion channels -- Post-embedding immunohistochemistry in the localization of receptors and ion channels -- High-Resolution localization of membrane proteins by SDS-digested freeze-fracture replica labelling (SDS-FRL) -- Application of virus vectors for anterograde tract-tracing and single-neuron labeling studies -- Analysis of synaptic connections using viral vectors at the electron microscopic level -- Morphological and neurochemical characterization of electrophysiologically identified cells -- Using electrophysiology to study synaptic and extrasynaptic ionotropic receptors in hippocampal neurons -- Biophysical methods to analyze direct G-protein regulation of neuronal voltage-gated calcium channels -- Electrophysiological recordings in behaving animals -- Voltammetry in behaving animals -- In vivo brain microdialysis of monoamines -- Calcium transients in single dendrites and spines of pyramidal neurons in vitro -- Dynamic recording of membrane potential from hippocampal neurons by using a FRET-based voltage biosensor -- Determination of GPCR-mediated cAMP accumulation in rat striatal synaptosomes -- GPCR-mediated MAPK/ERK cascade activation in brain slices -- Index.
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| 650 |
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0 |
|a Neurosciences.
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| 650 |
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0 |
|a Neurobiology.
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| 650 |
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|a Biology—Technique.
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| 650 |
1 |
4 |
|a Neurosciences.
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| 650 |
2 |
4 |
|a Neurobiology.
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| 650 |
2 |
4 |
|a Biological Techniques.
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| 700 |
1 |
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|a Luján, Rafael.
|e editor.
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| 700 |
1 |
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|a Ciruela, Francisco.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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