Receptor and Ion Channel Detection in the Brain : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2016.
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| Edición: | 1st ed. 2016. |
| Colección: | Neuromethods,
110 |
| Materias: |
Tabla de Contenidos:
- Preface
- Production of high-quality antibodies for the study of receptors and ion channels in brain
- Co-immunoprecipitation from brain
- Sub-synaptic membrane fractionation
- Investigation of neurotransmitter receptors in brain slices using cell surface biotinylation
- Single nanoparticle tracking of surface ion channels and receptors in brain cells
- Radioligand binding detection of receptors in brain membranes
- Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain
- Fluorescent ligands and TR-FRET to study receptor-receptor interactions in the brain
- In situ Proximity Ligation Assay to study and understand the distribution and balance of GPCR homo- and heteroreceptor complexes in the brain
- Fluorescent in situ hybridization for sensitive and specific labeling
- Autoradiographic visualization of G protein-coupled receptors in brain
- Analysis of the expression profile and regional distribution of neurotransmitter receptors and ion channels in the central nervous system using histoblots
- Immunohistochemistry for ion channels and their interacting molecules: Tips for improving antibody accessibility
- Localization of GFP-tagged proteins at the electron microscope
- Pre-embedding methods in the localization of receptors and ion channels
- Post-embedding immunohistochemistry in the localization of receptors and ion channels
- High-Resolution localization of membrane proteins by SDS-digested freeze-fracture replica labelling (SDS-FRL)
- Application of virus vectors for anterograde tract-tracing and single-neuron labeling studies
- Analysis of synaptic connections using viral vectors at the electron microscopic level
- Morphological and neurochemical characterization of electrophysiologically identified cells
- Using electrophysiology to study synaptic and extrasynaptic ionotropic receptors in hippocampal neurons
- Biophysical methods to analyze direct G-protein regulation of neuronal voltage-gated calcium channels
- Electrophysiological recordings in behaving animals
- Voltammetry in behaving animals
- In vivo brain microdialysis of monoamines
- Calcium transients in single dendrites and spines of pyramidal neurons in vitro
- Dynamic recording of membrane potential from hippocampal neurons by using a FRET-based voltage biosensor
- Determination of GPCR-mediated cAMP accumulation in rat striatal synaptosomes
- GPCR-mediated MAPK/ERK cascade activation in brain slices
- Index.