Noncanonical Amino Acids : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Lemke, Edward A. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:1st ed. 2018.
Colección:Methods in Molecular Biology, 1728
Materias:
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245 1 0 |a Noncanonical Amino Acids :  |b Methods and Protocols /  |c edited by Edward A. Lemke. 
250 |a 1st ed. 2018. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2018. 
300 |a XI, 411 p. 76 illus., 39 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
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490 1 |a Methods in Molecular Biology,  |v 1728 
505 0 |a Leveraging Formylglycine-Generating Enzyme for Production of Site-Specifically Modified Bioconjugates -- Artificial Division of Codon Boxes for Expansion of the Amino Acid Repertoire of Ribosomal Polypeptide Synthesis -- Cell-free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Using Cell Extracts from RF-1 deletion E. coli Strains -- Tub-tag Labeling; Chemoenzymatic Incorporation of Unnatural Amino Acids -- Directed Evolution of Orthogonal Pyrrolysyl-tRNA Synthetases in Escherichia coli for the Genetic Encoding of Noncanonical Amino Acids -- Genetic Code Expansion in Enteric Bacterial Pathogens -- Self-directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and its Incorporation into Model Proteins -- Residue-specific Incorporation of no Canonical Amino Acids for Protein Engineering -- Using Amber and Ochre Nonsense Codons to Code Two Different Noncanonical Amino Acids in One Protein Gene -- Genetic Incorporation of Unnatural Amino Acids into Proteins of Interest in Streptomyces venezuelae ATCC 15439 -- Expression and Purification of Site-specifically Lysine-acetylated and Natively-folded Proteins for Biophysical Investigations -- Site-specific Incorporation of Sulfotyrosine Using an Expanded Genetic Code -- Site-specific Protein Labeling With Tetrazine Amino Acids -- Mapping of Protein Interfaces in Live Cells Using Genetically Encoded Crosslinkers -- Generation of Stable Amber Suppression Cell Lines -- Trapping Chromatin Interacting Proteins with Genetically Encoded, UV-Activatable Crosslinkers In Vivo -- Genetically Encoding Unnatural Amino Acids in Neurons in vitro and in the Embryonic Mouse Brain for Optical Control of Neuronal Proteins -- Genetic Code Expansion-and Click Chemistry-Based Site--Specific Protein Labelling For Intracellular DNA--PAINT Imaging -- MultiBacTAG- Genetic Code Expansion using the Baculovirus Expression System in Sf21 Cells -- Production and Chemoselective Modification of Adeno-associated Virus Site-specifically Incorporating an Unnatural Amino Acid Residue into its Capsid -- Generation of Intramolecular FRET Probes via Noncanonical Amino Acid Mutagenesis -- Fluorogenic Tetrazine--siliconrhodamine Probe for the Labeling of Non-canonical Amino Acid Tagged Proteins -- Site-specific Protein Labeling Utilizing Lipoic Acid Ligase (LplA) and Bioorthogonal Inverse Electron Demand Diels-Alder Reaction -- Genetic Encoding of Unnatural Amino Acids in C. elegans. 
650 0 |a Biochemistry. 
650 1 4 |a Biochemistry, general. 
700 1 |a Lemke, Edward A.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
900 |a Libro descargado a ALEPH en bloque (proveniente de proveedor)