Noncanonical Amino Acids : Methods and Protocols /
Autor Corporativo: | |
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Otros Autores: | |
Formato: | eBook |
Lenguaje: | English |
Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2018.
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Edición: | 1st ed. 2018. |
Colección: | Methods in Molecular Biology,
1728 |
Materias: |
Tabla de Contenidos:
- Leveraging Formylglycine-Generating Enzyme for Production of Site-Specifically Modified Bioconjugates
- Artificial Division of Codon Boxes for Expansion of the Amino Acid Repertoire of Ribosomal Polypeptide Synthesis
- Cell-free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Using Cell Extracts from RF-1 deletion E. coli Strains
- Tub-tag Labeling; Chemoenzymatic Incorporation of Unnatural Amino Acids
- Directed Evolution of Orthogonal Pyrrolysyl-tRNA Synthetases in Escherichia coli for the Genetic Encoding of Noncanonical Amino Acids
- Genetic Code Expansion in Enteric Bacterial Pathogens
- Self-directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and its Incorporation into Model Proteins
- Residue-specific Incorporation of no Canonical Amino Acids for Protein Engineering
- Using Amber and Ochre Nonsense Codons to Code Two Different Noncanonical Amino Acids in One Protein Gene
- Genetic Incorporation of Unnatural Amino Acids into Proteins of Interest in Streptomyces venezuelae ATCC 15439
- Expression and Purification of Site-specifically Lysine-acetylated and Natively-folded Proteins for Biophysical Investigations
- Site-specific Incorporation of Sulfotyrosine Using an Expanded Genetic Code
- Site-specific Protein Labeling With Tetrazine Amino Acids
- Mapping of Protein Interfaces in Live Cells Using Genetically Encoded Crosslinkers
- Generation of Stable Amber Suppression Cell Lines
- Trapping Chromatin Interacting Proteins with Genetically Encoded, UV-Activatable Crosslinkers In Vivo
- Genetically Encoding Unnatural Amino Acids in Neurons in vitro and in the Embryonic Mouse Brain for Optical Control of Neuronal Proteins
- Genetic Code Expansion-and Click Chemistry-Based Site
- Specific Protein Labelling For Intracellular DNA
- PAINT Imaging
- MultiBacTAG- Genetic Code Expansion using the Baculovirus Expression System in Sf21 Cells
- Production and Chemoselective Modification of Adeno-associated Virus Site-specifically Incorporating an Unnatural Amino Acid Residue into its Capsid
- Generation of Intramolecular FRET Probes via Noncanonical Amino Acid Mutagenesis
- Fluorogenic Tetrazine
- siliconrhodamine Probe for the Labeling of Non-canonical Amino Acid Tagged Proteins
- Site-specific Protein Labeling Utilizing Lipoic Acid Ligase (LplA) and Bioorthogonal Inverse Electron Demand Diels-Alder Reaction
- Genetic Encoding of Unnatural Amino Acids in C. elegans.