RNA Detection : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Gaspar, Imre. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:1st ed. 2018.
Colección:Methods in Molecular Biology, 1649
Materias:
Tabla de Contenidos:
  • The Secret Life of RNA: Lessons from Emerging Methodologies
  • Quantification of 2'-O-Me Residues in RNA using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)
  • Identifying the m6A Methylome by Affinity Purification and Sequencing
  • PARIS: Psoralen Analysis of RNA Interactions and Structures with High Throughput and Resolution
  • Axon-TRAP-RiboTag: Affinity-Purification of Translated mRNAs from Neuronal Axons in Mouse In Vivo
  • LCM-Seq: A Method for Spatial Transcriptomic Profiling using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing
  • Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas
  • Single mRNA Molecule Detection in Drosophila
  • Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryos
  • Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction
  • Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections
  • Hybridization Chain Reaction for Direct mRNA Detection without Nucleic Acid Purification
  • In Situ Detection of MicroRNA Expression with RNAscope Probes
  • Padlock Probes to Detect Single Nucleotide Polymorphisms
  • Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons
  • Optimizing Molecular Beacons for Intracellular Analysis of RNA
  • Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH
  • In Vivo Visualization and Function Probing of Transport mRNPs using Injected FIT Probes
  • Visualizing RNA in Live Bacterial Cells using Fluorophore- and Quencher-Binding Aptamers
  • Method for Imaging Live-Cell RNA using an RNA Aptamer and a Fluorescent Probe
  • RNA Live Imaging in the Model Microorganism Ustilago maydis
  • Real-Time Fluorescence Imaging of Single-Molecule Endogenous Non-Coding RNA in Living Cells
  • Live Imaging of mRNA Synthesis in Drosophila
  • Imaging Newly Transcribed RNA in Cells by Using a Clickable Azide-Modified UTP Analog
  • Detection of the First Round of Translation: The TRICK Assay
  • Imaging Translation Dynamics of Single mRNA Molecules in Live Cells
  • Systematic Detection of Poly(A)+ RNA-Interacting Proteins and Their Differential Binding
  • Isolation and Characterization of Endogenous RNPs from Brain Tissues
  • Individual Nucleotide Resolution UV Cross-Linking and Immunoprecipitation (iCLIP) to Determine Protein-RNA Interactions
  • RNA Tagging: Preparation of High-Throughput Sequencing Libraries
  • RAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells.