Phospho-Proteomics : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: von Stechow, Louise. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Springer, 2016.
Edición:2nd ed. 2016.
Colección:Methods in Molecular Biology, 1355
Materias:
Tabla de Contenidos:
  • Thiol-ene Enabled Detection of Thiophosphorylation as a Labeling Strategy for Phosphoproteins
  • Phosphopeptide Detection with Biotin-Labeled Phos-tag
  • Phosphopeptide Enrichment by Covalent Chromatography after Solid Phase Derivatization of Protein Digests on Reversed Phase Supports
  • Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics
  • Identification of Direct Kinase Substrates Using Analogue-Sensitive Alleles
  • Quantitative Analysis of Tissue Samples by Combining iTRAQ Isobaric Labeling with Selected/Multiple Reaction Monitoring (SRM/MRM)
  • Enrichment Strategies in Phosphoproteomics
  • Phosphopeptide Enrichment by Immobilized Metal Affinity Chromatography
  • The use of Titanium Dioxide for Selective Enrichment of Phosphorylated Peptides
  • Sequential Elution from IMAC (SIMAC) – An Efficient Method for Enrichment and Separation of Mono- and Multi-Phosphorylated Peptides
  • Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and Fractionation
  • Offline High pH Reversed-Phase Peptide Fractionation for Deep Phosphoproteome Coverage
  • Phosphopeptide Enrichment Using Various Magnetic Nanocomposites – An Overview
  • Two Dimensional Gel Electrophoresis-Based Plant Phosphoproteomics
  • Variable Digestion Strategies for Phosphoproteomics Analysis
  • On-line LC-FAIMS-MS/MS for the Analysis of Phosphorylation in Proteins
  • Simple and Reproducible Sample Preparation for Single Shot Phosphoproteomics With High Sensitivity
  • Identification of Direct Kinase Substrates via Kinase Assay Linked Phosphoproteomics
  • Phosphoprotein Detection by High-Throughput Flow Cytometry
  • Resources for Assignment of Phosphorylation Sites on Peptides and Proteins
  • From Phosphosites to Kinases
  • Search Databases and Statistics – Pitfalls and Best Practices in Phosphoproteomics
  • Systems Analysis for Interpretation of Phosphoproteomics Data.