Protein Tyrosine Phosphatases : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2016.
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| Edition: | 1st ed. 2016. |
| Series: | Methods in Molecular Biology,
1447 |
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Table of Contents:
- The Extended Family of Protein Tyrosine Phosphatases
- Global RT-PCR and RT-qPCR Analysis of the mRNA Expression of the Human PTPome
- Expression, Purification, and Kinetic Analysis of PTP Domains
- Peptide Microarrays for Real Time Kinetic Profiling of Tyrosine Phosphatase Activity of Recombinant Phosphatases and Phosphatases in Lysates of Cells or Tissue Samples
- Tailor-Made Protein Tyrosine Phosphatases: In Vitro Site-Directed Mutagenesis of PTEN and PTPRZ-B
- Assays to Measure PTEN Lipid Phosphatase Activity In Vitro from Purified Enzyme or Immunoprecipitates
- Assessing the Biological Activity of the Glucan Phosphatase Laforin
- Discovery and Evaluation of PRL Trimer Disruptors for Novel Anticancer Agents
- Analyzing Pseudophosphatase Function
- Crystallization of PTP Domains
- NMR Spectroscopy to Study MAP Kinase Binding to MAP Kinase Phosphatases
- Visualizing and Quantitating the Spatiotemporal Regulation of Ras/ERK Signaling by Dual-Specificity Mitogen-Activated Protein Phosphatases (MKPs)
- In Situ Proximity Ligation Assay (In Situ PLA) to Assess PTP-Protein Interactions
- Use of Dominant Negative/Substrate Trapping PTP Mutations to Search for PTP Interactors/Substrates
- Detection and Identification of Ligands for Mammalian RPTP Extracellular Domains
- Production of Osteoclasts for Studying Protein Tyrosine Phosphatase Signaling
- Functional Analysis of Protein Tyrosine Phosphatases in Thrombosis and Haemostasis
- Functional Analysis of Dual-Specificity Protein Phosphatases in Angiogenesis
- Studying Protein-Tyrosine Phosphatases in Zebrafish
- Live staining of Drosophila Embryos with RPTP Fusion Proteins to Detect and Characterize Expression of Cell-Surface RPTP Ligands
- Methods to Study Protein Tyrosine Phosphatases Acting on Yeast MAPKs.