NLR Proteins : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Di Virgilio, Francesco. (Editor ), Pelegrín, Pablo. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1417
Materias:
LEADER 02479nam a22003015i 4500
001 000290872
005 20210930145235.0
007 cr nn 008mamaa
008 160524s2016 xxu| s |||| 0|eng d
020 |a 9781493935666 
024 7 |a 10.1007/978-1-4939-3566-6  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a NLR Proteins :  |b Methods and Protocols /  |c edited by Francesco Di Virgilio, Pablo Pelegrín. 
250 |a 1st ed. 2016. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2016. 
300 |a XI, 256 p. 52 illus., 43 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 1417 
505 0 |a Innate Immune Receptors -- Atypical Inflammasomes -- Assessment of Inflammasome Activation By Cytokine And Danger Signal Detection -- Investigating IL-1β Secretion Using Real-Time Single-Cell Imaging -- Measuring IL-1β Processing By Bioluminescence Sensors I: Using a Bioluminescence Resonance Energy Transfer Biosensor -- Measuring IL-1β Processing By Bioluminescence Sensors II: The Igluc System -- Assessing Extracellular ATP as Danger Signal In Vivo: The Pmeluc System -- Measuring NLR Oligomerization I: Size Exclusion Chromatography, Co-Immunoprecipitation and Cross-Linking -- Measuring NLR Oligomerization II: Detection of ASC Speck Formation by Confocal Microscopy and Immunofluorescence -- Measuring NLR Oligomerization III: Detection of NLRP3 Complex by Bioluminescence Resonance Energy Transfer -- Measuring NLR Oligomerization IV: Using Förster Resonance Energy Transfer (FRET)-Fluorescence Lifetime Imaging Microscopy (FLIM) to Determine the Close Proximity Of Inflammasome Components -- Measuring NLR Oligomerization V: In Situ Proximity Ligation Assay -- Assessing Caspase-1 Activation -- Cell-Free Assay for Inflammasome Activation -- Functional Reconstruction of Nlrs In HEK293 Cells -- Method to Measure Ubiquitinization of Nlrs -- Cytofluorometric Quantification of Cell Death Elicited By NLR Proteins -- NLR in Human Diseases: Role and Laboratory Findings. 
650 0 |a Proteins . 
650 1 4 |a Protein Science. 
700 1 |a Di Virgilio, Francesco.  |e editor. 
700 1 |a Pelegrín, Pablo.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
900 |a Libro descargado a ALEPH en bloque (proveniente de proveedor)