|
|
|
|
| LEADER |
03314nam a22002895i 4500 |
| 001 |
000291051 |
| 005 |
20201013141654.0 |
| 007 |
cr nn 008mamaa |
| 008 |
181013s2019 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781493987603
|
| 024 |
7 |
|
|a 10.1007/978-1-4939-8760-3
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a SNAREs :
|b Methods and Protocols /
|c edited by Rutilio Fratti.
|
| 250 |
|
|
|a 1st ed. 2019.
|
| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2019.
|
| 300 |
|
|
|a XIV, 407 p. 74 illus., 66 illus. in color. :
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in Molecular Biology,
|v 1860
|
| 505 |
0 |
|
|a Molecular Dynamics Simulations of the SNARE Complex -- Mesoscale Computational Modelling of Protein-membrane Interactions based on Continuum Mean-field Theory -- EPR Lineshape Analysis to IOnvestigate the SNARE Folding Intermediates -- Dynamic Light Scattering Analysis to Dissect Intermediates of SNARE-Mediated Membrane Fusion -- Kinetic and Thermodynamic Quantification of Protein Complex Assembly: The Example of SNAREpins -- Single-molecule Optical Tweezers Study of Regulated SNARE Assembly -- Studying Munc18:Syntaxin Interactions using Small-angle Scattering -- Using Force Spectroscopy to Probe Coiled-Coil Assembly and Membrane Fusion -- SNAP25 S-Guanylation and SNARE Complex Formation -- Analysis of the Role of Sec3 in SNARE Assembly and Membrane Fusion -- Use of Microscale Thermophoresis (MST) to Measure Binding Affinities of Components of the Fusion Machinery -- Use of Surface Plasmon Resonance (SPR) to Determine Binding Affinities and Kinetic Parameters Between Components Important in Fusion Machinery -- Determination of Sec18-Lipid Interactions by Liposome Binding Assay -- Using Nanodiscs to Probe Ca2+-dependent Membrane Interaction of Synaptotagmin-1 -- Functional reconstitution of Intracellular Vesicle Fusion using Purified SNAREs and Sec1/Munc18 (SM) Proteins -- Assay of Lipid Mixing and Fusion Pore Formation in the Fusion of Yeast Vacuoles -- A nanodisc-cell Fusion Assay with Single Pore Sensitivity and Sub-millisecond Time Resolution -- An In Vitro Assay of trans-SNARE Complex Formation during Yeast Vacuole Fusion using Epitope Tag-free SNAREs -- A Cell-Free Content Mixing Assay for SNARE-Mediated Multivesicular Body-Vacuole Membrane Fusion -- Reconstituted Proteoliposome Fusion Mediated by yeast SNARE-family Proteins -- Real-Time Fluorescence Detection of Calcium Efflux during Vacuolar Membrane Fusion -- Single Molecule Fluorescence Measurement of SNARE-mediated Vesicle Fusion -- Quantifying Intramolecular Protein Conformational Dynamics under Lipid Interaction using smFRET and FCCS -- Visualization of SNARE-Mediated Organelle Membrane Hemifusion By Electron Microscopy -- Studies of the Secretory Machinery Dynamics by Total Internal Reflection Fluorescence Microscopy in Bovine Adrenal Chromaffin Cells -- Imaging SNAP-29 in Drosophila.
|
| 650 |
|
0 |
|a Biochemistry.
|
| 650 |
1 |
4 |
|a Biochemistry, general.
|
| 700 |
1 |
|
|a Fratti, Rutilio.
|e editor.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|
| 900 |
|
|
|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|