SNAREs : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edición: | 1st ed. 2019. |
| Colección: | Methods in Molecular Biology,
1860 |
| Materias: |
Tabla de Contenidos:
- Molecular Dynamics Simulations of the SNARE Complex
- Mesoscale Computational Modelling of Protein-membrane Interactions based on Continuum Mean-field Theory
- EPR Lineshape Analysis to IOnvestigate the SNARE Folding Intermediates
- Dynamic Light Scattering Analysis to Dissect Intermediates of SNARE-Mediated Membrane Fusion
- Kinetic and Thermodynamic Quantification of Protein Complex Assembly: The Example of SNAREpins
- Single-molecule Optical Tweezers Study of Regulated SNARE Assembly
- Studying Munc18:Syntaxin Interactions using Small-angle Scattering
- Using Force Spectroscopy to Probe Coiled-Coil Assembly and Membrane Fusion
- SNAP25 S-Guanylation and SNARE Complex Formation
- Analysis of the Role of Sec3 in SNARE Assembly and Membrane Fusion
- Use of Microscale Thermophoresis (MST) to Measure Binding Affinities of Components of the Fusion Machinery
- Use of Surface Plasmon Resonance (SPR) to Determine Binding Affinities and Kinetic Parameters Between Components Important in Fusion Machinery
- Determination of Sec18-Lipid Interactions by Liposome Binding Assay
- Using Nanodiscs to Probe Ca2+-dependent Membrane Interaction of Synaptotagmin-1
- Functional reconstitution of Intracellular Vesicle Fusion using Purified SNAREs and Sec1/Munc18 (SM) Proteins
- Assay of Lipid Mixing and Fusion Pore Formation in the Fusion of Yeast Vacuoles
- A nanodisc-cell Fusion Assay with Single Pore Sensitivity and Sub-millisecond Time Resolution
- An In Vitro Assay of trans-SNARE Complex Formation during Yeast Vacuole Fusion using Epitope Tag-free SNAREs
- A Cell-Free Content Mixing Assay for SNARE-Mediated Multivesicular Body-Vacuole Membrane Fusion
- Reconstituted Proteoliposome Fusion Mediated by yeast SNARE-family Proteins
- Real-Time Fluorescence Detection of Calcium Efflux during Vacuolar Membrane Fusion
- Single Molecule Fluorescence Measurement of SNARE-mediated Vesicle Fusion
- Quantifying Intramolecular Protein Conformational Dynamics under Lipid Interaction using smFRET and FCCS
- Visualization of SNARE-Mediated Organelle Membrane Hemifusion By Electron Microscopy
- Studies of the Secretory Machinery Dynamics by Total Internal Reflection Fluorescence Microscopy in Bovine Adrenal Chromaffin Cells
- Imaging SNAP-29 in Drosophila.