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180823s2018 xxu| s |||| 0|eng d |
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|a 9781493986910
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024 |
7 |
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|a 10.1007/978-1-4939-8691-0
|2 doi
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040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
1 |
4 |
|a The LINC Complex :
|b Methods and Protocols /
|c edited by Gregg G. Gundersen, Howard J. Worman.
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250 |
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|a 1st ed. 2018.
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2018.
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300 |
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|a XIV, 338 p. 61 illus., 41 illus. in color. :
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 1840
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|a EM Tomography of LINC Complexes -- Recombinant Purification of the Periplasmic Portion of the LINC Complex -- Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis -- Analysis of Nesprin-2 Interaction with its Binding Partners and Actin -- Interactions of Nesprin-4-containing LINC Complexes in Outer Hair Cells Explored by BioID -- Using Nesprin Tension Sensors to Measure Force on the LINC Complex -- Analyzing Mechanotransduction through the LINC Complex in Isolated Nuclei -- Direct Force Probe for Nuclear Mechanics -- Centrifugal Displacement of Nuclei in Adherent Cells to Study LINC Complex Dependent Mechanisms of Homeostatic Nuclear Positioning -- Assembly and Use of a Microfluidic Device to Study Cell Migration in Confined Environments -- Investigating LINC Complex Protein Homo-oligomerization in the Nuclear Envelopes of Living Cells Using Fluorescence Fluctuation Spectroscopy -- Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super Resolution Imaging -- Genetic Analysis of Nuclear Migration and Anchorage to Study LINC Complexes During Development of Caenorhabditis elegans -- High-resolution Imaging Methods to Analyze LINC Complex Function During Drosophila Muscle Development -- Computational Methods for Studying the Plant Nucleus -- Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System -- Immunolabeling Protocols for Studying Meiosis in Plant Mutants Defective for Nuclear Envelope Components -- Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice -- An in vitro System to Measure the Positioning, Stiffness and Rupture of the Nucleus in Skeletal Muscle -- Functional Analysis of LINC Complexes in Skin -- Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer -- Next-generation Sequencing and Mutational Analysis: Implications for Genes Encoding LINC Complex Proteins.
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650 |
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|a Cell biology.
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650 |
1 |
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|a Cell Biology.
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700 |
1 |
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|a Gundersen, Gregg G.
|e editor.
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|a Worman, Howard J.
|e editor.
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710 |
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|a SpringerLink (Online service)
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773 |
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|t Springer eBooks
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900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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