The LINC Complex : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Gundersen, Gregg G. (Editor ), Worman, Howard J. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:1st ed. 2018.
Colección:Methods in Molecular Biology, 1840
Materias:
Tabla de Contenidos:
  • EM Tomography of LINC Complexes
  • Recombinant Purification of the Periplasmic Portion of the LINC Complex
  • Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis
  • Analysis of Nesprin-2 Interaction with its Binding Partners and Actin
  • Interactions of Nesprin-4-containing LINC Complexes in Outer Hair Cells Explored by BioID
  • Using Nesprin Tension Sensors to Measure Force on the LINC Complex
  • Analyzing Mechanotransduction through the LINC Complex in Isolated Nuclei
  • Direct Force Probe for Nuclear Mechanics
  • Centrifugal Displacement of Nuclei in Adherent Cells to Study LINC Complex Dependent Mechanisms of Homeostatic Nuclear Positioning
  • Assembly and Use of a Microfluidic Device to Study Cell Migration in Confined Environments
  • Investigating LINC Complex Protein Homo-oligomerization in the Nuclear Envelopes of Living Cells Using Fluorescence Fluctuation Spectroscopy
  • Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super Resolution Imaging
  • Genetic Analysis of Nuclear Migration and Anchorage to Study LINC Complexes During Development of Caenorhabditis elegans
  • High-resolution Imaging Methods to Analyze LINC Complex Function During Drosophila Muscle Development
  • Computational Methods for Studying the Plant Nucleus
  • Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System
  • Immunolabeling Protocols for Studying Meiosis in Plant Mutants Defective for Nuclear Envelope Components
  • Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice
  • An in vitro System to Measure the Positioning, Stiffness and Rupture of the Nucleus in Skeletal Muscle
  • Functional Analysis of LINC Complexes in Skin
  • Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer
  • Next-generation Sequencing and Mutational Analysis: Implications for Genes Encoding LINC Complex Proteins.