Skin Tissue Engineering : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edición: | 1st ed. 2019. |
| Colección: | Methods in Molecular Biology,
1993 |
| Materias: |
Tabla de Contenidos:
- Part 1 : Isolation and culture of skin cells
- Chap 1: Isolation and Culture of Human Keratinocytes
- Chap 2 : Promotion of Human Epidermal Keratinocyte Expansion in Feeder Cell Co-culture
- Chap 3 : Isolation and Culture of Epidermal Melanocytes
- Chap 4: Long-term Expansion of Mouse Primary Epidermal Keratinocytes Using a TGF-beta Signaling Inhibitor
- Chap 5 : Isolation and Culture of Hair Follicle Dermal Sheath Mesenchymal Stromal Cells
- Chap 6 : Isolation and Culture of Human Dermal Fibroblasts
- Chap 7: Isolation and Culture of Human Dermal Microvascular Endothelial Cells
- Chap 8: Isolation of Stromal Vascular Fraction by Fractionation of Adipose Tissue
- Part 2 : Tissue engineering skin and engineered skin models
- Chap 9 : Engineering a Multi-Layered Skin Equivalent: the Importance of Endogenous Extracellular Matrix Maturation to Provide Robustness and Reproducibility
- Chap 10 : Three-dimensional Epidermal Model from Human Hair follicle-derived Keratinocytes
- Chap 11 : Fabrication of a Co-Culture System with Human Sweat Gland Derived Cells and Peripheral Nerve Cells
- Chap 12 : Engineering a Multilayered Skin Substitute with Keratinocytes, Fibroblasts, Adipose Derived Stem Cells and Adipocytes
- Chap 13 : Skin Tissue Engineering Fabrication of Chimeric Hair Follicles for Skin Tissue Engineering
- Chap 14 : Isolation and Culture of Epidermolysis Bullosa Cells and Organotypic Skin Models
- Part 3 : In vitro and vivo models testing skin, skin cells, and tissue engineered skin
- Chap 15 : Effects of the Extracellular Matrix on the Proteome of Primary Skin Fibroblasts
- Chap 16 : Standard Preparation Protocol of Human Skin Samples for Transmission Electron Microscopy
- Chap 17 : Methods for Assessing Scaffold Vascualarization In Vivo
- Chap 18 : Human Reconstructed Skin in a Mouse Model
- Chap 19 : Pig Model to Test Tissue Engineered Skin
- Chap 20 : Transplantation of Autologous Dermo-Epidermal Skin Substitutes in a Pig Model.