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03196nam a22003135i 4500 |
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20201013141452.0 |
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cr nn 008mamaa |
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190530s2019 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493995202
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| 024 |
7 |
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|a 10.1007/978-1-4939-9520-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a SMC Complexes :
|b Methods and Protocols /
|c edited by Anjana Badrinarayanan.
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| 250 |
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|a 1st ed. 2019.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2019.
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| 300 |
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|a XIII, 336 p. 91 illus., 55 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 2004
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| 505 |
0 |
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|a Using Cell Cycle Restricted Alleles to Study the Chromatin Dynamics and Functions of the Structural Maintenance of Chromosome (SMC) Complexes In Vivo -- Degradation of S. cerevisiae Cohesin with the Auxin-Inducible Degron System -- Efficient Depletion of Fission Yeast Condensin by Combined Transcriptional Repression and Auxin-Induced Degradation -- Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts -- High-Throughput Allelic Replacement Screening in Bacillus subtilis -- Identifying Functional Domains in Subunits of Structural Maintenance of Chromosome (SMC) Complexes by Transposon Mutagenesis Screen in Yeast -- Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes -- Knocking in Multi-Functional Gene Tags into SMC Complex Subunits Using Gene Editing -- Chromosome Conformation Capture with Deep Sequencing to Study the Roles of the Structural Maintenance of Chromosomes Complex In Vivo -- Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin Immunoprecipitation -- Analysis of Cohesin Association to Newly Replicated DNA through Nascent Strand Binding Assay (NSBA) -- Preparation of Cell Cycle Synchronized Saccharomyces cerevisiae Cells for Hi-C -- Dissecting DNA Compaction by the Bacterial Condensin MukB -- In Vivo and In Vitro Assay for Monitoring the Topological Loading of Bacterial Condensins on DNA -- A Protocol for Assaying the ATPase Activity of Recombinant Cohesin Holocomplexes -- In Vitro Detection of Long Noncoding RNA Generated from DNA Double-Strand Breaks -- Tracking Bacterial Chromosome Dynamics with Microfluidics-Based Live Cell Imaging -- Live-Cell Fluorescence Imaging of RecN in Caulobacter crescentus under DNA Damage -- Microinjection Techniques in Fly Embryos to Study the Function and Dynamics of SMC Complexes -- Purification and Biophysical Characterization of the Mre11-Rad50-Nbs1 Complex -- Three-Dimensional Thermodynamic Simulation of Condensin as a DNA-Based Translocase -- Molecular Dynamics Simulations of Condensin-Mediated Mitotic Chromosome Assembly.
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| 650 |
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|a Genetics.
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| 650 |
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|a Proteins .
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| 650 |
1 |
4 |
|a Genetics and Genomics.
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| 650 |
2 |
4 |
|a Protein Science.
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| 700 |
1 |
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|a Badrinarayanan, Anjana.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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