SMC Complexes : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2019.
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| Edición: | 1st ed. 2019. |
| Colección: | Methods in Molecular Biology,
2004 |
| Materias: |
Tabla de Contenidos:
- Using Cell Cycle Restricted Alleles to Study the Chromatin Dynamics and Functions of the Structural Maintenance of Chromosome (SMC) Complexes In Vivo
- Degradation of S. cerevisiae Cohesin with the Auxin-Inducible Degron System
- Efficient Depletion of Fission Yeast Condensin by Combined Transcriptional Repression and Auxin-Induced Degradation
- Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts
- High-Throughput Allelic Replacement Screening in Bacillus subtilis
- Identifying Functional Domains in Subunits of Structural Maintenance of Chromosome (SMC) Complexes by Transposon Mutagenesis Screen in Yeast
- Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes
- Knocking in Multi-Functional Gene Tags into SMC Complex Subunits Using Gene Editing
- Chromosome Conformation Capture with Deep Sequencing to Study the Roles of the Structural Maintenance of Chromosomes Complex In Vivo
- Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin Immunoprecipitation
- Analysis of Cohesin Association to Newly Replicated DNA through Nascent Strand Binding Assay (NSBA)
- Preparation of Cell Cycle Synchronized Saccharomyces cerevisiae Cells for Hi-C
- Dissecting DNA Compaction by the Bacterial Condensin MukB
- In Vivo and In Vitro Assay for Monitoring the Topological Loading of Bacterial Condensins on DNA
- A Protocol for Assaying the ATPase Activity of Recombinant Cohesin Holocomplexes
- In Vitro Detection of Long Noncoding RNA Generated from DNA Double-Strand Breaks
- Tracking Bacterial Chromosome Dynamics with Microfluidics-Based Live Cell Imaging
- Live-Cell Fluorescence Imaging of RecN in Caulobacter crescentus under DNA Damage
- Microinjection Techniques in Fly Embryos to Study the Function and Dynamics of SMC Complexes
- Purification and Biophysical Characterization of the Mre11-Rad50-Nbs1 Complex
- Three-Dimensional Thermodynamic Simulation of Condensin as a DNA-Based Translocase
- Molecular Dynamics Simulations of Condensin-Mediated Mitotic Chromosome Assembly.