The ESCRT Complexes : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Culetto, Emmanuel. (Editor ), Legouis, Renaud. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 1998
Materias:
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245 1 4 |a The ESCRT Complexes :  |b Methods and Protocols /  |c edited by Emmanuel Culetto, Renaud Legouis. 
250 |a 1st ed. 2019. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2019. 
300 |a XIV, 322 p. 72 illus., 43 illus. in color. :  |b online resource. 
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338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 1998 
505 0 |a Analysis of the Archaeal ESCRT Apparatus -- Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster -- Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc -- Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative-Light Electron Microscopy -- Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells -- Immuno-Localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy -- Single Cell Fluorescence Ratio Image Analysis for Studying ESCRT Function in Receptor Trafficking -- Genetic and Biochemical Analyses of Yeast ESCRT -- Live Imaging of ESCRT Proteins in Microfluidically-Isolated Hippocampal Axons -- Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High Resolution Imaging Techniques SIM and Cryo-SXT -- Three Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software -- Transient Expression of ESCRT-Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts -- Crystallization and Biophysical Approaches for Studying the Interactions between the Vps4-MIT Domain and ESCRT-III Proteins -- Biochemical Approaches to Studying C. elegans ESCRT Function In Vitro -- Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays -- Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting -- Purification of Plant ESCRT Proteins for Polyclonal Antibody Production -- Genetic and Cytological Methods to Study ESCRT Cell Cycle Function in Fission Yeast -- ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis -- Genetic Suppressor Screen Using an Inducible FREE1-RNAi Line to Detect ESCRT Genetic Interactors in Arabidopsis thaliana -- Screening of Interactions with the ESCRT Machinery by a Gaussia princeps Split Luciferase-Based Complementation Assay -- RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells. 
650 0 |a Cell membranes . 
650 0 |a Proteins . 
650 0 |a Cell physiology. 
650 1 4 |a Membrane Biology. 
650 2 4 |a Protein Science. 
650 2 4 |a Cell Physiology. 
700 1 |a Culetto, Emmanuel.  |e editor. 
700 1 |a Legouis, Renaud.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
900 |a Libro descargado a ALEPH en bloque (proveniente de proveedor)