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190627s2019 xxu| s |||| 0|eng d |
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|a 9781493994922
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|a 10.1007/978-1-4939-9492-2
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a The ESCRT Complexes :
|b Methods and Protocols /
|c edited by Emmanuel Culetto, Renaud Legouis.
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|a 1st ed. 2019.
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2019.
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|a XIV, 322 p. 72 illus., 43 illus. in color. :
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 1998
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|a Analysis of the Archaeal ESCRT Apparatus -- Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster -- Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc -- Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative-Light Electron Microscopy -- Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells -- Immuno-Localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy -- Single Cell Fluorescence Ratio Image Analysis for Studying ESCRT Function in Receptor Trafficking -- Genetic and Biochemical Analyses of Yeast ESCRT -- Live Imaging of ESCRT Proteins in Microfluidically-Isolated Hippocampal Axons -- Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High Resolution Imaging Techniques SIM and Cryo-SXT -- Three Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software -- Transient Expression of ESCRT-Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts -- Crystallization and Biophysical Approaches for Studying the Interactions between the Vps4-MIT Domain and ESCRT-III Proteins -- Biochemical Approaches to Studying C. elegans ESCRT Function In Vitro -- Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays -- Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting -- Purification of Plant ESCRT Proteins for Polyclonal Antibody Production -- Genetic and Cytological Methods to Study ESCRT Cell Cycle Function in Fission Yeast -- ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis -- Genetic Suppressor Screen Using an Inducible FREE1-RNAi Line to Detect ESCRT Genetic Interactors in Arabidopsis thaliana -- Screening of Interactions with the ESCRT Machinery by a Gaussia princeps Split Luciferase-Based Complementation Assay -- RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells.
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|a Cell membranes .
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|a Proteins .
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|a Cell physiology.
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|a Membrane Biology.
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|a Protein Science.
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|a Cell Physiology.
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|a Culetto, Emmanuel.
|e editor.
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|a Legouis, Renaud.
|e editor.
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|a SpringerLink (Online service)
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|t Springer eBooks
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900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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