The ESCRT Complexes : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Culetto, Emmanuel. (Editor ), Legouis, Renaud. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2019.
Edición:1st ed. 2019.
Colección:Methods in Molecular Biology, 1998
Materias:
Tabla de Contenidos:
  • Analysis of the Archaeal ESCRT Apparatus
  • Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster
  • Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc
  • Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative-Light Electron Microscopy
  • Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells
  • Immuno-Localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy
  • Single Cell Fluorescence Ratio Image Analysis for Studying ESCRT Function in Receptor Trafficking
  • Genetic and Biochemical Analyses of Yeast ESCRT
  • Live Imaging of ESCRT Proteins in Microfluidically-Isolated Hippocampal Axons
  • Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High Resolution Imaging Techniques SIM and Cryo-SXT
  • Three Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software
  • Transient Expression of ESCRT-Components in Arabidopsis Root Cell Suspension Culture-Derived Protoplasts
  • Crystallization and Biophysical Approaches for Studying the Interactions between the Vps4-MIT Domain and ESCRT-III Proteins
  • Biochemical Approaches to Studying C. elegans ESCRT Function In Vitro
  • Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays
  • Assessment of ESCRT Protein CHMP5 Activity on Client Protein Ubiquitination by Immunoprecipitation and Western Blotting
  • Purification of Plant ESCRT Proteins for Polyclonal Antibody Production
  • Genetic and Cytological Methods to Study ESCRT Cell Cycle Function in Fission Yeast
  • ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis
  • Genetic Suppressor Screen Using an Inducible FREE1-RNAi Line to Detect ESCRT Genetic Interactors in Arabidopsis thaliana
  • Screening of Interactions with the ESCRT Machinery by a Gaussia princeps Split Luciferase-Based Complementation Assay
  • RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells.