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100301s2008 xxu| s |||| 0|eng d |
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|a 9781597451819
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| 024 |
7 |
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|a 10.1007/978-1-59745-181-9
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a SARS- and Other Coronaviruses :
|b Laboratory Protocols /
|c edited by Dave Cavanagh.
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| 250 |
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|a 1st ed. 2008.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2008.
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| 300 |
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|a XV, 326 p. 64 illus. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 454
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| 505 |
0 |
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|a Detection and Discovery of Coronaviruses -- A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses -- Detection of Group 1 Coronaviruses in Bats Using Universal Coronavirus Reverse Transcription Polymerase Chain Reactions -- Detection and Sequence Characterization of the 3?-End of Coronavirus Genomes Harboring the Highly Conserved RNA Motif s2m -- RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot -- Detection of Group 2a Coronaviruses with Emphasis on Bovine and Wild Ruminant Strains -- Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions -- Detection of New Viruses by VIDISCA -- Isolation, Growth, Titration, and Purification of Coronaviruses -- Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay -- The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration -- Isolation and Propagation of Coronaviruses inEmbryonated Eggs -- Large-Scale Preparation of UV-Inactivated SARS Coronavirus Virions for Vaccine Antigen -- Structure of Covs Analyzed by Electron Microscopy -- Purification and Electron Cryomicroscopy of Coronavirus Particles -- Expression of Coronavirus Proteins and Crystallization -- Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization -- Raising Antibodies to Coronavirus Proteins -- Generating Antibodies to the Gene 3 Proteins of Infectious Bronchitis Virus -- Establishment and Characterization of Monoclonal Antibodies Against SARS Coronavirus -- Production of Monospecific Rabbit Antisera Recognizing Nidovirus Proteins -- Manipulating the Genomes of Coronaviruses -- Manipulation of the Coronavirus Genome Using Targeted RNA Recombination with Interspecies Chimeric Coronaviruses -- Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAs -- Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses -- Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes -- Systematic Assembly and Genetic Manipulation of the Mouse Hepatitis Virus A59 Genome -- Identifying Coronavirus Receptors -- Identification of Sugar Residues Involved in the Binding of TGEV to Porcine Brush Border Membranes -- Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike Proteins.
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| 650 |
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0 |
|a Medical microbiology.
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| 650 |
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0 |
|a Infectious diseases.
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| 650 |
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0 |
|a Virology.
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| 650 |
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0 |
|a Laboratory medicine.
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| 650 |
1 |
4 |
|a Medical Microbiology.
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| 650 |
2 |
4 |
|a Infectious Diseases.
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| 650 |
2 |
4 |
|a Virology.
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| 650 |
2 |
4 |
|a Laboratory Medicine.
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| 700 |
1 |
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|a Cavanagh, Dave.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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