SARS- and Other Coronaviruses : Laboratory Protocols /
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2008.
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| Edition: | 1st ed. 2008. |
| Series: | Methods in Molecular Biology,
454 |
| Subjects: |
Table of Contents:
- Detection and Discovery of Coronaviruses
- A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses
- Detection of Group 1 Coronaviruses in Bats Using Universal Coronavirus Reverse Transcription Polymerase Chain Reactions
- Detection and Sequence Characterization of the 3?-End of Coronavirus Genomes Harboring the Highly Conserved RNA Motif s2m
- RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot
- Detection of Group 2a Coronaviruses with Emphasis on Bovine and Wild Ruminant Strains
- Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions
- Detection of New Viruses by VIDISCA
- Isolation, Growth, Titration, and Purification of Coronaviruses
- Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay
- The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
- Isolation and Propagation of Coronaviruses inEmbryonated Eggs
- Large-Scale Preparation of UV-Inactivated SARS Coronavirus Virions for Vaccine Antigen
- Structure of Covs Analyzed by Electron Microscopy
- Purification and Electron Cryomicroscopy of Coronavirus Particles
- Expression of Coronavirus Proteins and Crystallization
- Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization
- Raising Antibodies to Coronavirus Proteins
- Generating Antibodies to the Gene 3 Proteins of Infectious Bronchitis Virus
- Establishment and Characterization of Monoclonal Antibodies Against SARS Coronavirus
- Production of Monospecific Rabbit Antisera Recognizing Nidovirus Proteins
- Manipulating the Genomes of Coronaviruses
- Manipulation of the Coronavirus Genome Using Targeted RNA Recombination with Interspecies Chimeric Coronaviruses
- Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAs
- Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses
- Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
- Systematic Assembly and Genetic Manipulation of the Mouse Hepatitis Virus A59 Genome
- Identifying Coronavirus Receptors
- Identification of Sugar Residues Involved in the Binding of TGEV to Porcine Brush Border Membranes
- Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike Proteins.