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180921s2018 xxu| s |||| 0|eng d |
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|a 9781493987061
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| 024 |
7 |
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|a 10.1007/978-1-4939-8706-1
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
4 |
|a The Ubiquitin Proteasome System :
|b Methods and Protocols /
|c edited by Thibault Mayor, Gary Kleiger.
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| 250 |
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|a 1st ed. 2018.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2018.
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| 300 |
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|a XV, 413 p. 68 illus., 39 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1844
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| 505 |
0 |
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|a Characterization of RING-Between-RING E3 Ubiquitin Transfer Mechanisms -- Single Turnover RING/U-Box E3-Mediated Lysine Discharge Assays -- Methods for NAD-Dependent Ubiquitination Catalyzed by Legionella pneumophila Effector Proteins -- Using In Vitro Ubiquitylation Assays to Estimate the Affinities of Ubiquitin-Conjugating Enzymes for their Ubiquitin Ligase Partners -- Competition Assay for Measuring Deubiquitinating Enzyme Substrate Affinity -- Enzymatic Assembly of Ubiquitin Chains -- Ubiquitin-Activated Interaction Traps (UBAITs): Tools for Capturing Protein-Protein Interactions -- Generating Intracellular Modulators of E3 Ligases and Deubiquitinases from Phage-Displayed Ubiquitin Variant Libraries -- Integrated Proteogenomic Approach for Identifying Degradation Motifs in Eukaryotic Cells -- A Method to Monitor Protein Turnover by Flow Cytometry and to Screen for Factors that Control Degradation by Fluorescence-Activated Cell Sorting -- E. coli Based Selection and Expression Systems for Discovery, Characterization, and Purification of Ubiquitylated Proteins -- Strategies to Trap Enzyme-Substrate Complexes that Mimic Michaelis Intermediates during E3-Mediated Ubiquitin-Like Protein Ligation -- Small-Angle X-Ray Scattering for the Study of Proteins in the Ubiquitin Pathway -- Methods for Preparing Cryo-EM Grids of Large Macromolecular Complexes -- Recombinant Expression, Unnatural Amino-Acid Incorporation, and Site-Specific Labeling of 26S Proteasomal Subcomplexes -- Native Gel Approaches in Studying Proteasome Assembly and Chaperones -- Measuring the Overall Rate of Protein Breakdown in Cells and the Contributions of the Ubiquitin-Proteasome and Autophagy-Lysosomal Pathways -- Methods to Rapidly Prepare Mammalian 26S Proteasomes for Biochemical Analysis -- Measurement of the Multiple Activities of 26S Proteasomes -- Exploring the Regulation of Proteasome Function by Subunit Phosphorylation -- Scalable In Vitro Proteasome Activity Assay -- Exploring the Rampant Expansion of Ubiquitin Proteomics -- Ubiquitin diGLY Proteomics as an Approach to Identify and Quantify the Ubiquitin-Modified Proteome -- Interpreting the Language of Polyubiquitin with Linkage-Specific Antibodies and Mass Spectrometry -- Dissecting Dynamic and Heterogeneous Proteasome Complexes Using In Vivo Cross-Linking Assisted Affinity Purification and Mass Spectrometry.
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| 650 |
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|a Proteins .
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| 650 |
1 |
4 |
|a Protein Science.
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| 700 |
1 |
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|a Mayor, Thibault.
|e editor.
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| 700 |
1 |
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|a Kleiger, Gary.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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