The Ubiquitin Proteasome System : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2018.
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| Edición: | 1st ed. 2018. |
| Colección: | Methods in Molecular Biology,
1844 |
| Materias: |
Tabla de Contenidos:
- Characterization of RING-Between-RING E3 Ubiquitin Transfer Mechanisms
- Single Turnover RING/U-Box E3-Mediated Lysine Discharge Assays
- Methods for NAD-Dependent Ubiquitination Catalyzed by Legionella pneumophila Effector Proteins
- Using In Vitro Ubiquitylation Assays to Estimate the Affinities of Ubiquitin-Conjugating Enzymes for their Ubiquitin Ligase Partners
- Competition Assay for Measuring Deubiquitinating Enzyme Substrate Affinity
- Enzymatic Assembly of Ubiquitin Chains
- Ubiquitin-Activated Interaction Traps (UBAITs): Tools for Capturing Protein-Protein Interactions
- Generating Intracellular Modulators of E3 Ligases and Deubiquitinases from Phage-Displayed Ubiquitin Variant Libraries
- Integrated Proteogenomic Approach for Identifying Degradation Motifs in Eukaryotic Cells
- A Method to Monitor Protein Turnover by Flow Cytometry and to Screen for Factors that Control Degradation by Fluorescence-Activated Cell Sorting
- E. coli Based Selection and Expression Systems for Discovery, Characterization, and Purification of Ubiquitylated Proteins
- Strategies to Trap Enzyme-Substrate Complexes that Mimic Michaelis Intermediates during E3-Mediated Ubiquitin-Like Protein Ligation
- Small-Angle X-Ray Scattering for the Study of Proteins in the Ubiquitin Pathway
- Methods for Preparing Cryo-EM Grids of Large Macromolecular Complexes
- Recombinant Expression, Unnatural Amino-Acid Incorporation, and Site-Specific Labeling of 26S Proteasomal Subcomplexes
- Native Gel Approaches in Studying Proteasome Assembly and Chaperones
- Measuring the Overall Rate of Protein Breakdown in Cells and the Contributions of the Ubiquitin-Proteasome and Autophagy-Lysosomal Pathways
- Methods to Rapidly Prepare Mammalian 26S Proteasomes for Biochemical Analysis
- Measurement of the Multiple Activities of 26S Proteasomes
- Exploring the Regulation of Proteasome Function by Subunit Phosphorylation
- Scalable In Vitro Proteasome Activity Assay
- Exploring the Rampant Expansion of Ubiquitin Proteomics
- Ubiquitin diGLY Proteomics as an Approach to Identify and Quantify the Ubiquitin-Modified Proteome
- Interpreting the Language of Polyubiquitin with Linkage-Specific Antibodies and Mass Spectrometry
- Dissecting Dynamic and Heterogeneous Proteasome Complexes Using In Vivo Cross-Linking Assisted Affinity Purification and Mass Spectrometry.