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cr nn 008mamaa |
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180910s2018 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493987047
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| 024 |
7 |
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|a 10.1007/978-1-4939-8704-7
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
4 |
|a The CRAC Channel :
|b Methods and Protocols /
|c edited by Aubin Penna, Bruno Constantin.
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| 250 |
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|a 1st ed. 2018.
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| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2018.
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| 300 |
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|a XI, 204 p. 41 illus., 26 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1843
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| 505 |
0 |
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|a Patch-Clamp Recording of the CRAC Channel Current in STIM-Orai Overexpressing Cells -- Fluorescence-Based Ratiometric Measurement of CRAC Channel Activity in STIM-Orai Overexpressing HEK-293 Cells -- Recording SOCE Activity in Neurons by Patch-Clamp Electrophysiology and Microfluorometric Calcium Imaging -- Mn2+ Quenching Assay for Store-Operated Calcium Entry -- Fluorescence-Based Measurements of Store-Operated Ca2+ Entry in Cancer Cells using Fluo-4 and Confocal Live Cell Imaging -- Fluorescence-Based Measurements of the CRAC Channel Activity in Cell Populations -- Indirect Measurement of CRAC Channel Activity using NFAT Nuclear Translocation by Flow Cytometry in Jurkat Cells -- CRAC Channel Components Quantitative Expression (In Tissues and Cell Lines) using qPCR -- Western-Blotting and Co-Immunoprecipitation of Endogenous STIM/ORAI and Protein Partners -- Study of Endogenous CRAC Channels in Human Mast Cells using an Adenoviral Delivery System to Transduce Cells with Orai-Targeting shRNAs or with cDNAs Expressing Dominant-Negative Orai Channel Mutations -- Store-Operated Ca2+ Entry in Drosophila Primary Neuron Cultures -- Study of the Endogenous CRAC Channel using shRNA-Mediated Gene Silencing -- Engineered Cross-Linking to Study the Pore Architecture of the CRAC Channel -- Measurement of the CRAC Channel Fast Ca2+-Dependent Inactivation (FCDI) -- High Resolution Imaging of STIM/Orai Subcellular Localization using Array Confocal Laser Scanning Microscopy -- Single Channel Single Molecule Detection (SC-SMD) System.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 700 |
1 |
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|a Penna, Aubin.
|e editor.
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| 700 |
1 |
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|a Constantin, Bruno.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|