The CRAC Channel : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2018.
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| Edición: | 1st ed. 2018. |
| Colección: | Methods in Molecular Biology,
1843 |
| Materias: |
Tabla de Contenidos:
- Patch-Clamp Recording of the CRAC Channel Current in STIM-Orai Overexpressing Cells
- Fluorescence-Based Ratiometric Measurement of CRAC Channel Activity in STIM-Orai Overexpressing HEK-293 Cells
- Recording SOCE Activity in Neurons by Patch-Clamp Electrophysiology and Microfluorometric Calcium Imaging
- Mn2+ Quenching Assay for Store-Operated Calcium Entry
- Fluorescence-Based Measurements of Store-Operated Ca2+ Entry in Cancer Cells using Fluo-4 and Confocal Live Cell Imaging
- Fluorescence-Based Measurements of the CRAC Channel Activity in Cell Populations
- Indirect Measurement of CRAC Channel Activity using NFAT Nuclear Translocation by Flow Cytometry in Jurkat Cells
- CRAC Channel Components Quantitative Expression (In Tissues and Cell Lines) using qPCR
- Western-Blotting and Co-Immunoprecipitation of Endogenous STIM/ORAI and Protein Partners
- Study of Endogenous CRAC Channels in Human Mast Cells using an Adenoviral Delivery System to Transduce Cells with Orai-Targeting shRNAs or with cDNAs Expressing Dominant-Negative Orai Channel Mutations
- Store-Operated Ca2+ Entry in Drosophila Primary Neuron Cultures
- Study of the Endogenous CRAC Channel using shRNA-Mediated Gene Silencing
- Engineered Cross-Linking to Study the Pore Architecture of the CRAC Channel
- Measurement of the CRAC Channel Fast Ca2+-Dependent Inactivation (FCDI)
- High Resolution Imaging of STIM/Orai Subcellular Localization using Array Confocal Laser Scanning Microscopy
- Single Channel Single Molecule Detection (SC-SMD) System.