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20221007105831.0 |
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100301s2004 xxu| s |||| 0|eng d |
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|a 9781592597468
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| 024 |
7 |
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|a 10.1385/1592597467
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Ribozymes and siRNA protocols /
|c edited by Mouldy Sioud.
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| 250 |
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|a 2nd ed. 2004.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2004.
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| 300 |
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|a XVI, 624 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 252
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| 505 |
0 |
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|a Ribozyme- and siRNA-Mediated mRNA Degradation -- Combination of Chemical and Enzymatic RNA Synthesis -- Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes -- Real-Time Monitoring of RNA and DNA Reactions by Fluorescence Detection -- Quantification of Ribozyme Target RNA Using Real-Time PCR -- Analysis of Ribozyme Structure and Function by Nucleotide Analog Interference Mapping -- Analysis of Global Conformational Transitions in Ribozymes -- In Vivo Detection of Ribozyme Cleavage Products and RNA Structure by Use of Terminal Transferase-Dependent PCR -- Identification of Efficient Cleavage Sites in Long-Target RNAs -- Selection In Vitro of Allosteric Ribozymes -- Regulation of Ribozyme Cleavage Activity by Oligonucleotides -- Tetracycline-Regulated Expression of Hammerhead Ribozymes In Vivo -- Ribozyme Expression Systems -- Design and Expression of Chimeric U1/Ribozyme Transgenes -- Design and Validation of Therapeutic Hammerhead Ribozymes for Autosomal Dominant Diseases -- Helicase-Attached Novel Hybrid Ribozymes -- Functional Gene Discovery Using Hybrid Ribozyme Libraries -- Maxizyme Technology -- Target-Site Selection for the 10-23 DNAzyme -- In Vitro Selected RNA-Cleaving DNA Enzymes From Combinatorial Libraries -- Nucleic Acid Sequence Analysis Using DNAzymes -- Crystallization of the Hairpin Ribozyme -- An Experimental Method for Selecting Effective Target Sites and Designing Hairpin Ribozymes -- Design and Optimization of Sequence-Specific Hairpin Ribozymes -- Design, Targeting, and Initial Screening of sTRSV-Derived Hairpin Ribozymes for Optimum Helix 1 Length and Catalytic Efficiency In Vitro -- Optimization and Application of the Group I Ribozyme Trans-Splicing Reaction -- Repair of Myotonic Dystrophy Protein Kinase (DMPK) Transcripts by Trans-Splicing Ribozymes -- General Design and Construction of RNase P Ribozymes for Gene-Targeting Applications -- In Vitro Selection of RNase P Ribozymes That Efficiently Cleave a Target mRNA -- In Vitro Selection of External Guide Sequences for Directing Human RNase P to Cleave a Target mRNA -- RNase P-Mediated Inhibition of Viral Growth by Exogenous Administration of Short Oligonucleotide External Guide Sequence -- RNase P Ribozyme As an Antiviral Agent Against Human Cytomegalovirus -- Inhibition of Gene Expression by Nucleic Acid Enzymes in Rodent Models of Human Disease -- Potential Design Rules and Enzymatic Synthesis of siRNAs -- RNA Interference (RNAi) With RNase III-Prepared siRNAs -- RNAi Expression Vectors in Mammalian Cells -- Gene-Array Analysis of Glioma Cells After Treatment With an Anti-PKC? siRNA -- RNAi in Living Mice -- Construction and Transfection of PCR Products Expressing siRNAs or shRNAs in Mammalian Cells -- Systemic Delivery of Synthetic siRNAs -- Adenovirus-Delivered siRNA -- RNAi Expression Vectors in Plant Cells -- Delivery Agents for Oligonucleotides -- Selection of Peptides for Specific Delivery of Oligonucleotides Into Cancer Cells -- Clinical Gene Therapy Research Utilizing Ribozymes -- Critical Steps in the Implementation of Hematopoietic Progenitor-Cell Gene Therapy Using Ribozyme Vectors.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Sioud, Mouldy.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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