Ribozymes and siRNA protocols /
| Corporate Author: | |
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2004.
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| Edition: | 2nd ed. 2004. |
| Series: | Methods in Molecular Biology,
252 |
| Subjects: |
Table of Contents:
- Ribozyme- and siRNA-Mediated mRNA Degradation
- Combination of Chemical and Enzymatic RNA Synthesis
- Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes
- Real-Time Monitoring of RNA and DNA Reactions by Fluorescence Detection
- Quantification of Ribozyme Target RNA Using Real-Time PCR
- Analysis of Ribozyme Structure and Function by Nucleotide Analog Interference Mapping
- Analysis of Global Conformational Transitions in Ribozymes
- In Vivo Detection of Ribozyme Cleavage Products and RNA Structure by Use of Terminal Transferase-Dependent PCR
- Identification of Efficient Cleavage Sites in Long-Target RNAs
- Selection In Vitro of Allosteric Ribozymes
- Regulation of Ribozyme Cleavage Activity by Oligonucleotides
- Tetracycline-Regulated Expression of Hammerhead Ribozymes In Vivo
- Ribozyme Expression Systems
- Design and Expression of Chimeric U1/Ribozyme Transgenes
- Design and Validation of Therapeutic Hammerhead Ribozymes for Autosomal Dominant Diseases
- Helicase-Attached Novel Hybrid Ribozymes
- Functional Gene Discovery Using Hybrid Ribozyme Libraries
- Maxizyme Technology
- Target-Site Selection for the 10-23 DNAzyme
- In Vitro Selected RNA-Cleaving DNA Enzymes From Combinatorial Libraries
- Nucleic Acid Sequence Analysis Using DNAzymes
- Crystallization of the Hairpin Ribozyme
- An Experimental Method for Selecting Effective Target Sites and Designing Hairpin Ribozymes
- Design and Optimization of Sequence-Specific Hairpin Ribozymes
- Design, Targeting, and Initial Screening of sTRSV-Derived Hairpin Ribozymes for Optimum Helix 1 Length and Catalytic Efficiency In Vitro
- Optimization and Application of the Group I Ribozyme Trans-Splicing Reaction
- Repair of Myotonic Dystrophy Protein Kinase (DMPK) Transcripts by Trans-Splicing Ribozymes
- General Design and Construction of RNase P Ribozymes for Gene-Targeting Applications
- In Vitro Selection of RNase P Ribozymes That Efficiently Cleave a Target mRNA
- In Vitro Selection of External Guide Sequences for Directing Human RNase P to Cleave a Target mRNA
- RNase P-Mediated Inhibition of Viral Growth by Exogenous Administration of Short Oligonucleotide External Guide Sequence
- RNase P Ribozyme As an Antiviral Agent Against Human Cytomegalovirus
- Inhibition of Gene Expression by Nucleic Acid Enzymes in Rodent Models of Human Disease
- Potential Design Rules and Enzymatic Synthesis of siRNAs
- RNA Interference (RNAi) With RNase III-Prepared siRNAs
- RNAi Expression Vectors in Mammalian Cells
- Gene-Array Analysis of Glioma Cells After Treatment With an Anti-PKC? siRNA
- RNAi in Living Mice
- Construction and Transfection of PCR Products Expressing siRNAs or shRNAs in Mammalian Cells
- Systemic Delivery of Synthetic siRNAs
- Adenovirus-Delivered siRNA
- RNAi Expression Vectors in Plant Cells
- Delivery Agents for Oligonucleotides
- Selection of Peptides for Specific Delivery of Oligonucleotides Into Cancer Cells
- Clinical Gene Therapy Research Utilizing Ribozymes
- Critical Steps in the Implementation of Hematopoietic Progenitor-Cell Gene Therapy Using Ribozyme Vectors.