Ribozymes and siRNA protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Sioud, Mouldy. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
Edition:2nd ed. 2004.
Series:Methods in Molecular Biology, 252
Subjects:
Table of Contents:
  • Ribozyme- and siRNA-Mediated mRNA Degradation
  • Combination of Chemical and Enzymatic RNA Synthesis
  • Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes
  • Real-Time Monitoring of RNA and DNA Reactions by Fluorescence Detection
  • Quantification of Ribozyme Target RNA Using Real-Time PCR
  • Analysis of Ribozyme Structure and Function by Nucleotide Analog Interference Mapping
  • Analysis of Global Conformational Transitions in Ribozymes
  • In Vivo Detection of Ribozyme Cleavage Products and RNA Structure by Use of Terminal Transferase-Dependent PCR
  • Identification of Efficient Cleavage Sites in Long-Target RNAs
  • Selection In Vitro of Allosteric Ribozymes
  • Regulation of Ribozyme Cleavage Activity by Oligonucleotides
  • Tetracycline-Regulated Expression of Hammerhead Ribozymes In Vivo
  • Ribozyme Expression Systems
  • Design and Expression of Chimeric U1/Ribozyme Transgenes
  • Design and Validation of Therapeutic Hammerhead Ribozymes for Autosomal Dominant Diseases
  • Helicase-Attached Novel Hybrid Ribozymes
  • Functional Gene Discovery Using Hybrid Ribozyme Libraries
  • Maxizyme Technology
  • Target-Site Selection for the 10-23 DNAzyme
  • In Vitro Selected RNA-Cleaving DNA Enzymes From Combinatorial Libraries
  • Nucleic Acid Sequence Analysis Using DNAzymes
  • Crystallization of the Hairpin Ribozyme
  • An Experimental Method for Selecting Effective Target Sites and Designing Hairpin Ribozymes
  • Design and Optimization of Sequence-Specific Hairpin Ribozymes
  • Design, Targeting, and Initial Screening of sTRSV-Derived Hairpin Ribozymes for Optimum Helix 1 Length and Catalytic Efficiency In Vitro
  • Optimization and Application of the Group I Ribozyme Trans-Splicing Reaction
  • Repair of Myotonic Dystrophy Protein Kinase (DMPK) Transcripts by Trans-Splicing Ribozymes
  • General Design and Construction of RNase P Ribozymes for Gene-Targeting Applications
  • In Vitro Selection of RNase P Ribozymes That Efficiently Cleave a Target mRNA
  • In Vitro Selection of External Guide Sequences for Directing Human RNase P to Cleave a Target mRNA
  • RNase P-Mediated Inhibition of Viral Growth by Exogenous Administration of Short Oligonucleotide External Guide Sequence
  • RNase P Ribozyme As an Antiviral Agent Against Human Cytomegalovirus
  • Inhibition of Gene Expression by Nucleic Acid Enzymes in Rodent Models of Human Disease
  • Potential Design Rules and Enzymatic Synthesis of siRNAs
  • RNA Interference (RNAi) With RNase III-Prepared siRNAs
  • RNAi Expression Vectors in Mammalian Cells
  • Gene-Array Analysis of Glioma Cells After Treatment With an Anti-PKC? siRNA
  • RNAi in Living Mice
  • Construction and Transfection of PCR Products Expressing siRNAs or shRNAs in Mammalian Cells
  • Systemic Delivery of Synthetic siRNAs
  • Adenovirus-Delivered siRNA
  • RNAi Expression Vectors in Plant Cells
  • Delivery Agents for Oligonucleotides
  • Selection of Peptides for Specific Delivery of Oligonucleotides Into Cancer Cells
  • Clinical Gene Therapy Research Utilizing Ribozymes
  • Critical Steps in the Implementation of Hematopoietic Progenitor-Cell Gene Therapy Using Ribozyme Vectors.