Therapeutic Applications of Ribozymes and Riboswitches : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Lafontaine, Daniel. (Editor ), Dubé, Audrey. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1103
Materias:
Tabla de Contenidos:
  • Identification of Regulatory RNA in Bacterial Genomes by Genome-Scale Mapping of Transcription Start Sites
  • Screening Inhibitory potential of anti-HIV RT RNA Aptamers
  • Design and Evaluation of Clinically Relevant SOFA-HDV Ribozymes Targeting HIV RNA
  • Directing RNase P-mediated Cleavage of Target mRNAs by Engineered External Guide Sequences in Cultured Cells
  • Design and Analysis of Hammerhead Ribozyme Activity Against an Artificial Gene Target
  • Knockdown Strategies for the Study of Proprotein Converstase and Proliferation in Prostate Cancer Cells
  • Use of Tumor-Targeting Trans-Splicing Ribozyme for Cancer Treatment
  • Characterization of Hairpin Ribozyme Reactions
  • Finding Instances of Riboswitches and Ribozymes by Homology Search of Structured RNA with Infernal
  • Structure-based Virtual Screening for the Identification of RNA-binding Ligands
  • Probing Riboswitch Binding Sites with Molecular Docking, Focused Libraries, and in-line Probing Assays
  • Discovery of Small Molecule Modifiers of microRNAs for the Treatment of HCV Infection
  • Bacterial Flavin Mononucleotide Riboswitches as Targets for Flavin Analogs
  • Construction and Application of Riboswitch-based Sensors That Detect Metabolites Within Bacterial Cells
  • Screening Assays to Identify Artificial glmS Ribozyme Activators
  • Analysis of Riboswitch Structure and Ligand Binding using Small-angle X-ray Scattering (SAXS)
  • Use of SHAPE to Select 2AP Substitution sites for RNA-ligand Interactions and Dynamics Studies
  • Cell Internalization SELEX:  In Vitro Selection for Molecules that Internalize into Cells
  • DNA Electronic Switches Based on Analyte-Responsive Aptamers.