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04047nam a22003255i 4500 |
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000291571 |
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20210607093950.0 |
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120810s2012 xxu| s |||| 0|eng d |
020 |
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|a 9781617799686
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024 |
7 |
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|a 10.1007/978-1-61779-968-6
|2 doi
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040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
1 |
0 |
|a Single Domain Antibodies :
|b Methods and Protocols /
|c edited by Dirk Saerens, Serge Muyldermans.
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250 |
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|a 1st ed. 2012.
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260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2012.
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300 |
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|a XV, 580 p. 89 illus., 35 illus. in color. :
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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337 |
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|a computer
|b c
|2 rdamedia
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338 |
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|a online resource
|b cr
|2 rdacarrier
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490 |
1 |
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|a Methods in Molecular Biology,
|v 911
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505 |
0 |
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|a From Whole Monoclonal Antibodies to Single Domain Antibodies: Think Small -- Introduction to Heavy Chain Antibodies and Derived Nanobodies -- Overview and Discovery of IgNARs and Generation of VNARs -- Creation of the Large and Highly Functional Synthetic Repertoire of Human VH and Vκ Domain Antibodies -- Preparation of a Naïve Library of Camelid Single Domain Antibodies -- Selection by Phage Display of Single Domain Antibodies Specific to Antigens in Their Native Conformation -- Semi-Automated Panning of Naive Camelidae Libraries and Selection of Single-Domain Antibodies Against Peptide Antigens -- Pichia Surface Display: A Tool for Screening Single Domain Antibodies -- Bacterial Two Hybrid: A Versatile One-Step Intracellular Selection Method -- Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies -- Selection of Functional Single Domain Antibody Fragments for Interfering with Protein-Protein Interactions Inside Cells: A “One Plasmid” Mammalian Two-Hybrid System -- Cell-Free Selection of Domain Antibodies by In Vitro Compartmentalization -- Selection of VHHs Under Application Conditions -- Isolation and Characterization of Clostridium difficile Toxin-Specific Single-Domain Antibodies -- Selection of VHH Antibody Fragments that Recognize Different Aβ Depositions Using Complex Immune Libraries -- Expression of Single-Domain Antibodies in Bacterial Systems -- Expression of VHHs in S. cerevisiae -- Stable Expression of Chimeric Heavy Chain Antibodies in CHO Cells -- Production of Camel-Like Antibodies in Plants -- Selecting and Purifying Autonomous Human Variable Heavy (VH) Domains -- Solubility and Stability Engineering of Human VH Domains -- Improvement of Proteolytic Stability through In Silico Engineering -- Selection of Human VH Single Domains with Improved Biophysical Properties by Phage Display -- Improvement of Single Domain Antibody Stability by Disulfide Bond Introduction -- Characterization of Single-Domain Antibodies with an Engineered Disulfide Bond -- Affinity Maturation of Single-Domain Antibodies by Yeast Surface Display -- Multivalent Display of Single-Domain Antibodies -- Methods for Determining the PK Parameters of AlbudAbs and of Long Serum Half Life Drugs Made Using the AlbudAb Technology -- Fluorescent Protein Specific Nanotraps to Study Protein-Protein Interactions and Histone-Tail Peptide Binding -- Site-Specific Labeling of His-Tagged Nanobodies with 99mTc: A Practical Guide -- Nanobody-Based Chromatin Immunoprecipitation -- User-Friendly Expression Plasmids Enable the Fusion of VHHs to Application-Specific Tags -- Application of Single Domain Antibodies in Tumor Histochemistry -- Nanobodies as Structural Probes of Protein Misfolding and Fibril Formation -- Molecular Imaging Using Nanobodies: A Case Study -- Case Study on Live Cell Apoptosis-Assay Using Lamin-Chromobody Cell-Lines for High-Content Analysis.
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650 |
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0 |
|a Immunology.
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650 |
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0 |
|a Antibodies.
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650 |
1 |
4 |
|a Immunology.
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650 |
2 |
4 |
|a Antibodies.
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700 |
1 |
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|a Saerens, Dirk.
|e editor.
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700 |
1 |
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|a Muyldermans, Serge.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
0 |
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|t Springer eBooks
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900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|