Single Domain Antibodies : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2012.
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| Edición: | 1st ed. 2012. |
| Colección: | Methods in Molecular Biology,
911 |
| Materias: |
Tabla de Contenidos:
- From Whole Monoclonal Antibodies to Single Domain Antibodies: Think Small
- Introduction to Heavy Chain Antibodies and Derived Nanobodies
- Overview and Discovery of IgNARs and Generation of VNARs
- Creation of the Large and Highly Functional Synthetic Repertoire of Human VH and Vκ Domain Antibodies
- Preparation of a Naïve Library of Camelid Single Domain Antibodies
- Selection by Phage Display of Single Domain Antibodies Specific to Antigens in Their Native Conformation
- Semi-Automated Panning of Naive Camelidae Libraries and Selection of Single-Domain Antibodies Against Peptide Antigens
- Pichia Surface Display: A Tool for Screening Single Domain Antibodies
- Bacterial Two Hybrid: A Versatile One-Step Intracellular Selection Method
- Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies
- Selection of Functional Single Domain Antibody Fragments for Interfering with Protein-Protein Interactions Inside Cells: A “One Plasmid” Mammalian Two-Hybrid System
- Cell-Free Selection of Domain Antibodies by In Vitro Compartmentalization
- Selection of VHHs Under Application Conditions
- Isolation and Characterization of Clostridium difficile Toxin-Specific Single-Domain Antibodies
- Selection of VHH Antibody Fragments that Recognize Different Aβ Depositions Using Complex Immune Libraries
- Expression of Single-Domain Antibodies in Bacterial Systems
- Expression of VHHs in S. cerevisiae
- Stable Expression of Chimeric Heavy Chain Antibodies in CHO Cells
- Production of Camel-Like Antibodies in Plants
- Selecting and Purifying Autonomous Human Variable Heavy (VH) Domains
- Solubility and Stability Engineering of Human VH Domains
- Improvement of Proteolytic Stability through In Silico Engineering
- Selection of Human VH Single Domains with Improved Biophysical Properties by Phage Display
- Improvement of Single Domain Antibody Stability by Disulfide Bond Introduction
- Characterization of Single-Domain Antibodies with an Engineered Disulfide Bond
- Affinity Maturation of Single-Domain Antibodies by Yeast Surface Display
- Multivalent Display of Single-Domain Antibodies
- Methods for Determining the PK Parameters of AlbudAbs and of Long Serum Half Life Drugs Made Using the AlbudAb Technology
- Fluorescent Protein Specific Nanotraps to Study Protein-Protein Interactions and Histone-Tail Peptide Binding
- Site-Specific Labeling of His-Tagged Nanobodies with 99mTc: A Practical Guide
- Nanobody-Based Chromatin Immunoprecipitation
- User-Friendly Expression Plasmids Enable the Fusion of VHHs to Application-Specific Tags
- Application of Single Domain Antibodies in Tumor Histochemistry
- Nanobodies as Structural Probes of Protein Misfolding and Fibril Formation
- Molecular Imaging Using Nanobodies: A Case Study
- Case Study on Live Cell Apoptosis-Assay Using Lamin-Chromobody Cell-Lines for High-Content Analysis.