Synthetic Protein Switches : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2017.
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| Edition: | 1st ed. 2017. |
| Series: | Methods in Molecular Biology,
1596 |
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Table of Contents:
- Synthetic Protein Switches: Theoretical and Experimental Considerations
- Construction of Allosteric Protein Switches by Alternate Frame Folding and Intermolecular Fragment Exchange
- Construction of Protein Switches by Domain Insertion and Directed Evolution
- Catalytic Amyloid Fibrils that Bind Copper to Activate Oxygen
- Ancestral Protein Reconstruction and Circular Permutation for Improving the Stability and Dynamic Range of FRET Sensors
- Method for Developing Optical Sensors Using a Synthetic Dye-Fluorescent Protein FRET Pair and Computational Modelling and Assessment
- Rational Design and Applications of Semi-Synthetic Modular Biosensors: SNIFITs and LUCIDs
- Ultrasensitive Firefly Luminescent Intermediate-Based Protein-Protein Interaction Assay (FlimPIA) Based on the Functional Complementation of Mutant Firefly Luciferases
- Quantitative and Dynamic Imaging of ATM Kinase Activity
- Creation of Antigen-Dependent β-Lactamase Fusion Protein Tethered by Circularly Permuted Antibody Variable Domains
- Protein and Protease Sensing by Allosteric Derepression
- DNA-Specific Biosensors Based on Intramolecular β-Lactamase-Inhibitor Complex Formation
- Engineering and Characterizing Synthetic Protease Sensors and Switches
- Characterizing Dynamic Protein-Protein Interactions Using the Genetically Encoded Split Biosensor Assay Technique Split TEV
- Development of a Synthetic Switch to Control Protein Stability in Eukaryotic Cells with Light
- Light-Regulated Protein Kinases Based on the CRY2-CIB1 System
- Yeast-Based Screening System for the Selection of Functional Light-Driven K+ Channels
- Primer-Aided Truncation for the Creation of Hybrid Proteins
- Engineering Small Molecule Responsive Split-Protein Kinases
- Directed Evolution Methods to Rewire Signaling Networks.