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160908s2016 xxu| s |||| 0|eng d |
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|a 9781493964635
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| 024 |
7 |
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|a 10.1007/978-1-4939-6463-5
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
4 |
|a The Golgi Complex :
|b Methods and Protocols /
|c edited by William J. Brown.
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| 250 |
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|a 1st ed. 2016.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2016.
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| 300 |
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|a XI, 219 p. 44 illus., 33 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1496
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| 505 |
0 |
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|a 4D Confocal Imaging of Yeast Organelles -- Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons -- Imaging Golgi Outposts in Fixed and Living Neurons -- Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor -- STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells -- Reconstitution of COPI Vesicle and Tubule Formation -- Reconstitution of Phospholipase A2-Dependent Golgi Membrane Tubules -- Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures -- High Content Analysis of the Golgi Complex by Correlative Screening Microscopy -- Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo -- In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in Adherent Mammalian Cell Cultures -- Creating Knock-Outs of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function -- Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway -- Assays to Study the Fragmentation of the Golgi Complex During the G2/M Transition of the Cell Cycle -- The Role of Lysophospholipid Acyltransferases in the Golgi Complex -- Methods to Purify and Assay Secretory Pathway Kinases.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Brown, William J.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|