The Golgi Complex : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2016.
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| Edition: | 1st ed. 2016. |
| Series: | Methods in Molecular Biology,
1496 |
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Table of Contents:
- 4D Confocal Imaging of Yeast Organelles
- Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons
- Imaging Golgi Outposts in Fixed and Living Neurons
- Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor
- STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells
- Reconstitution of COPI Vesicle and Tubule Formation
- Reconstitution of Phospholipase A2-Dependent Golgi Membrane Tubules
- Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures
- High Content Analysis of the Golgi Complex by Correlative Screening Microscopy
- Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo
- In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in Adherent Mammalian Cell Cultures
- Creating Knock-Outs of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function
- Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway
- Assays to Study the Fragmentation of the Golgi Complex During the G2/M Transition of the Cell Cycle
- The Role of Lysophospholipid Acyltransferases in the Golgi Complex
- Methods to Purify and Assay Secretory Pathway Kinases.