The Golgi Complex : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook | 
| Lenguaje: | English | 
| Publicado: | 
      New York, NY :
        Springer New York : Imprint: Humana,
    
      2016.
     | 
| Edición: | 1st ed. 2016. | 
| Colección: | Methods in Molecular Biology,
              1496             | 
| Materias: | 
                Tabla de Contenidos: 
            
                  - 4D Confocal Imaging of Yeast Organelles
 - Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons
 - Imaging Golgi Outposts in Fixed and Living Neurons
 - Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor
 - STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells
 - Reconstitution of COPI Vesicle and Tubule Formation
 - Reconstitution of Phospholipase A2-Dependent Golgi Membrane Tubules
 - Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures
 - High Content Analysis of the Golgi Complex by Correlative Screening Microscopy
 - Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo
 - In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in Adherent Mammalian Cell Cultures
 - Creating Knock-Outs of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function
 - Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway
 - Assays to Study the Fragmentation of the Golgi Complex During the G2/M Transition of the Cell Cycle
 - The Role of Lysophospholipid Acyltransferases in the Golgi Complex
 - Methods to Purify and Assay Secretory Pathway Kinases.