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160908s2016 xxu| s |||| 0|eng d |
| 020 |
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|a 9781493963584
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| 024 |
7 |
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|a 10.1007/978-1-4939-6358-4
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a SUMO :
|b Methods and Protocols /
|c edited by Manuel S. Rodriguez.
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| 250 |
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|a 1st ed. 2016.
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| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2016.
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| 300 |
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|a XI, 306 p. 54 illus., 34 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1475
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| 505 |
0 |
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|a Concepts and Methodologies to Study Protein SUMOylation: An Overview -- The Regulation of Chromatin by Dynamic SUMO Modifications -- Reconstitution of the Recombinant RanBP1 SUMO E3 Ligase Complex -- Production and Purification of Recombinant SUMOylated Proteins Using Engineered Bacteria -- A Fluorescent In Vitro Assay to Investigate Paralog Specific SUMO Conjugation -- Identification and Characterization of SUMO-SIM Interactions -- Real-Time Surface Plasmon Resonance (SPR) for the Analysis of Interactions between SUMO-Traps and Mono or PolySUMO Moieties -- Using Biotinylated SUMO-Traps to Analyze SUMOylated Proteins -- In Vitro Characterization of Chain Depolymerization Activities of SUMO-Specific Proteases -- Detection of Protein SUMOylation In Situ by Proximity Ligation Assays -- In Situ SUMOylation and DeSUMOylation Assays: Fluorescent Methods to Visualize SUMOylation and DeSUMOylation in Permeabilized Cells -- Analysis of SUMOylated Proteins in Cells and In Vivo Using BioSUMO Strategy -- Label-Free Identification and Quantification of SUMO Target Proteins -- The Use of Multimeric Protein Scaffolds for Identifying Multi-SUMO Binding Proteins -- Isolation of In Vivo SUMOylated Chromatin-Bound Proteins -- Identification of Substrates of Protein-Group SUMOylation -- Tools to Study SUMO Conjugation in Caenorhabditis elegans -- Purification of SUMO Conjugates from Arabidopsis for Mass Spectrometry Analysis -- Detection of SUMOylation in Plasmodium falciparum -- Systematic Localization and Identification of SUMOylation Substrates in Knock-In Mice Expressing Affinity-Tagged SUMO1.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 700 |
1 |
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|a Rodriguez, Manuel S.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|