SUMO : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2016.
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| Edition: | 1st ed. 2016. |
| Series: | Methods in Molecular Biology,
1475 |
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Table of Contents:
- Concepts and Methodologies to Study Protein SUMOylation: An Overview
- The Regulation of Chromatin by Dynamic SUMO Modifications
- Reconstitution of the Recombinant RanBP1 SUMO E3 Ligase Complex
- Production and Purification of Recombinant SUMOylated Proteins Using Engineered Bacteria
- A Fluorescent In Vitro Assay to Investigate Paralog Specific SUMO Conjugation
- Identification and Characterization of SUMO-SIM Interactions
- Real-Time Surface Plasmon Resonance (SPR) for the Analysis of Interactions between SUMO-Traps and Mono or PolySUMO Moieties
- Using Biotinylated SUMO-Traps to Analyze SUMOylated Proteins
- In Vitro Characterization of Chain Depolymerization Activities of SUMO-Specific Proteases
- Detection of Protein SUMOylation In Situ by Proximity Ligation Assays
- In Situ SUMOylation and DeSUMOylation Assays: Fluorescent Methods to Visualize SUMOylation and DeSUMOylation in Permeabilized Cells
- Analysis of SUMOylated Proteins in Cells and In Vivo Using BioSUMO Strategy
- Label-Free Identification and Quantification of SUMO Target Proteins
- The Use of Multimeric Protein Scaffolds for Identifying Multi-SUMO Binding Proteins
- Isolation of In Vivo SUMOylated Chromatin-Bound Proteins
- Identification of Substrates of Protein-Group SUMOylation
- Tools to Study SUMO Conjugation in Caenorhabditis elegans
- Purification of SUMO Conjugates from Arabidopsis for Mass Spectrometry Analysis
- Detection of SUMOylation in Plasmodium falciparum
- Systematic Localization and Identification of SUMOylation Substrates in Knock-In Mice Expressing Affinity-Tagged SUMO1.