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150319s2015 xxu| s |||| 0|eng d |
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|a 9781493925476
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|a 10.1007/978-1-4939-2547-6
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Small Non-Coding RNAs :
|b Methods and Protocols /
|c edited by Mathieu Rederstorff.
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| 250 |
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|a 1st ed. 2015.
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| 260 |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2015.
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| 300 |
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|a XI, 238 p. 35 illus., 15 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 1296
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| 505 |
0 |
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|a Small Non-Coding RNAs: A Quick Look in the Rear-View Mirror -- Alcoholic Precipitation of Small Non-Coding RNAs -- Quantification and Quality Control of a Small Non-Coding RNA Preparation -- Impact of RNA Isolation Protocols on RNA Detection by Northern Blotting -- Improved Northern Blot Detection of Small RNAs Using EDC Crosslinking and DNA/LNA Probes -- Direct Cloning of Double Stranded RNAs -- Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation -- Fluorescence in situ Hybridization of Small Non-Coding RNAs -- RT-qPCR Based Quantification of Small Non-Coding RNAs -- Stem-Loop RT-PCR Based Quantification of Small Non-Coding RNAs -- miR-RACE: An Effective Approach to Accurately Determine the Sequence of Computationally Identified miRNAs -- Probing Small Non-Coding RNAs Structures -- cDNA Library Generation for the Analysis of Small RNAs by High Throughput Sequencing -- CLIP-seq to Discover Transcriptome-Wide Imprinting of RNA Binding Proteins in Living Cells -- Microarray Analysis of Small Non-Coding RNAs -- RLM-RACE, PPM-RACE and qRT-PCR, an Integrated Strategy to Accurately Validate miRNA Target Genes -- Dual Luciferase Gene Reporter Assays to Study miRNA Function -- Gene Expression Knockdown by Transfection of siRNAs into Mammalian Cells -- Efficient and Selective Knockdown of Small Non-Coding RNAs -- Cell-SELEX: In Vitro Selection of Synthetic Small Specific Ligands -- Small Non-Coding RNAs and Aptamers in Diagnostics and Therapeutics.
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| 650 |
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|a Human genetics.
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| 650 |
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4 |
|a Human Genetics.
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| 700 |
1 |
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|a Rederstorff, Mathieu.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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