Small Non-Coding RNAs : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Edition: | 1st ed. 2015. |
| Series: | Methods in Molecular Biology,
1296 |
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Table of Contents:
- Small Non-Coding RNAs: A Quick Look in the Rear-View Mirror
- Alcoholic Precipitation of Small Non-Coding RNAs
- Quantification and Quality Control of a Small Non-Coding RNA Preparation
- Impact of RNA Isolation Protocols on RNA Detection by Northern Blotting
- Improved Northern Blot Detection of Small RNAs Using EDC Crosslinking and DNA/LNA Probes
- Direct Cloning of Double Stranded RNAs
- Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation
- Fluorescence in situ Hybridization of Small Non-Coding RNAs
- RT-qPCR Based Quantification of Small Non-Coding RNAs
- Stem-Loop RT-PCR Based Quantification of Small Non-Coding RNAs
- miR-RACE: An Effective Approach to Accurately Determine the Sequence of Computationally Identified miRNAs
- Probing Small Non-Coding RNAs Structures
- cDNA Library Generation for the Analysis of Small RNAs by High Throughput Sequencing
- CLIP-seq to Discover Transcriptome-Wide Imprinting of RNA Binding Proteins in Living Cells
- Microarray Analysis of Small Non-Coding RNAs
- RLM-RACE, PPM-RACE and qRT-PCR, an Integrated Strategy to Accurately Validate miRNA Target Genes
- Dual Luciferase Gene Reporter Assays to Study miRNA Function
- Gene Expression Knockdown by Transfection of siRNAs into Mammalian Cells
- Efficient and Selective Knockdown of Small Non-Coding RNAs
- Cell-SELEX: In Vitro Selection of Synthetic Small Specific Ligands
- Small Non-Coding RNAs and Aptamers in Diagnostics and Therapeutics.