The Wilms' Tumor (WT1) Gene : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Hastie, Nicholas. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1467
Materias:
Tabla de Contenidos:
  • WT1 Mutation in Childhood Cancer
  • WT1 and Renal Disease
  • The Role of WT1 in Embryonic Development and Normal Organ Homeostasis
  • Tools and Techniques for WT1-Based Lineage Tracing
  • Biological Systems and Methods for Studying WT1 in the Epicardium
  • Isolation and Colony Formation of Murine Bone and Bone Marrow Cells
  • Isolation and Fluorescent Activated Cell Sorting of Murine WT1-Expressing Adipocyte Precursor Cells
  • In Vivo Assays for Assessing the Role of the Wilms’ Tumor Suppressor 1 (WT1) in Angiogenesis
  • Multiphoton Microscopy for Visualizing Lipids in Tissue
  • Function and Regulation of the Wilm’s Tumor Suppressor 1 (WT1) Gene in Fish
  • Immunofluorescence Staining of WT1 on Sections of Zebrafish Embryos and Larvae
  • Fluorescence Activated Cell Sorting (FACS) Protocol for Podocyte Isolation in Adult Zebrafish
  • In Vitro Transcription to Study WT1 Function
  • Measuring Equilibrium Binding Constants for the WT1-DNA Interaction Using a Filter Binding Assay
  • Identifying Direct Downstream Targets: WT1 ChIP-Seq Analysis
  • WT1 Associated Protein-Protein Interaction Networks
  • Methods to Identify and Validate WT1-RNA Interaction
  • Bioinformatic Analysis of Next Generation Sequencing Data to Identify WT1 Associated Differential Gene and Isoform Expression
  • Immunotherapy Targeting WT1: Designing a Protocol for WT1 Peptide-Based Cancer Vaccine.