Transposons and Retrotransposons : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Garcia Perez, Jose Luis. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2016.
Edition:1st ed. 2016.
Series:Methods in Molecular Biology, 1400
Subjects:
Table of Contents:
  • Study of Transposable Elements and their Genomic Impact
  • Bacterial Group II Introns: Identification and Mobility Assay
  • In silico Methods to Identify Exapted Transposable Element Families
  • Retrotransposon Capture Sequencing (RC-seq): A Targeted, High-Throughput Approach to Resolve Somatic L1 Retrotransposition in Humans
  • Long Interspersed Element Sequencing (L1-Seq): A Method to Identify Somatic LINE-1 Insertions in the Human Genome
  • Combining Amplification Typing of L1 Active Subfamilies (ATLAS) with High Throughput Sequencing
  • RNA-seq Analysis to Measure the Expression of SINE Retroelements
  • Qualitative and Quantitative Assays of Transposition and Homologous Recombination of the Retrotransposon Tf1 in Schizosaccharomyces pombe
  • LINE Retrotransposition Assays in Saccharomyces cerevisiae
  • LINE-1 Cultured Cell Retrotransposition Assay
  • L1 Retrotransposition in Neural Progenitor Cells
  • Characterization of Engineered L1 Retrotransposition Events: The Recovery Method
  • SINE Retrotransposition: Evaluation of Alu Activity and Recovery of de novo Inserts
  • The Engineered SVA Trans-Mobilization Assay
  • Detection of LINE-1 RNAs by Northern Blot
  • Monitoring Long Interspersed Nuclear Element 1 Expression during Mouse Embryonic Stem Cells Differentiation
  • Immuno-Detection of Human LINE-1 Expression in Cultured Cells and Human Tissues
  • Cellular Localization of Engineered Human LINE-1 RNA and Proteins
  • Purification of L1-ribonucleoprotein Particles (L1-RNPs) from Cultured Human Cells
  • Characterization of L1-RiboNucleoprotein Particles
  • LEAP: L1 Element Amplification Protocol
  • Biochemical Approaches to Study LINE-1 Reverse Transcriptase Activity In Vitro
  • Methylated DNA Immunoprecipitation Analysis of Mammalian Endogenous Retroviruses
  • Profiling DNA Methylation and Hydroxymethylation at Retrotransposable Elements
  • A Large-Scale Functional Screen to Identify Epigenetic Repressors of Retrotransposon Expression
  • Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells with Sleeping Beauty Transposon-based Stable Gene Delivery. .