Subtilisin Enzymes : Practical Protein Engineering /
| Corporate Author: | |
|---|---|
| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer US : Imprint: Springer,
1996.
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| Edition: | 1st ed. 1996. |
| Series: | Advances in Experimental Medicine and Biology,
379 |
| Subjects: |
Table of Contents:
- I. Structural Studies of Subtilisin Enzymes Alone and in Complex with Inhibitors
- 1. The Complex between Mesentericopeptidase and Eglin-C
- 2. Crystallographic Study of Eglin-C Binding to Thermitase
- 3. Design of Specific Peptide Structures and Subtilisin Enzyme Inhibitors Using ?, ß-Dehydro-Residues
- 4. Structure and Function of Subtilisin BPN' as Studied through Crystallographic Studies on a Series of Its Complexes with Genetically Engineered Proteinaceous Inhibitor SSI
- 5. Three Dimensional Structure of the Antibiotic Bacitracin A Complexed to Two Different Subtilisin Proteases: Novel Mode of Enzyme Inhibition
- 6. Active Site Binding Loop Stabilization in the Subtilisin Inhibitor Eglin C: Structural and Functional Studies on Specifically Designed Mutants in Complex with Subtilisin and the Uncomplexed Inhibitor
- 7. Crystal Strucutures of the Alkaline Proteases Savinase and Esperase from Bacillus lentus
- II. New Enzymes
- 8. Modelling and Engineering of Enzyme/Substrate Interactions in Subtilisin-like Enzymes of Unknown 3-Dimensional Structure
- 9. Substilases: Subtilisin-like Serine Proteases
- 10. Cloning and Expression of the Gene Encoding a Novel Proteinase from Tritirachium album Limber
- III. Structure-Function Studies
- 11. Studies of Binding Sites in the Subtilisin from Bacillus lentus by Means of Site Directed Mutagenesis and Kinetic Investigations
- 12. Laundry Performance of Subtilisin Proteases
- 13. Free Energy Perturbation Techniques Applied to Subtilisin BPN' Stability
- IV. Biophysical Probes and Mutagenesis
- 14. Thermitase-Kinetic Differentiation to the Subtilisins
- 15. Fluorescence Decay of Tryptophans in Serine Proteinases from Microorganisms: Relation to X-Ray Models
- 16. Propeptide-Mediated Folding in Subtilisin: The Intramolecular Chaperone Concept
- 17. Roles of the N- and C-Terminal Pro-Sequences of Aqualysin I Precursor in the Processing and Extracellular Secretion of the Enzyme
- 18. Crystal Structure Analysis of Subtilisin BPN' Mutants Engineered for Studying Thermal Stability
- 19. Random Mutagenesis of the Weak Calcium Binding Site in Subtilisin Carlsberg and Screening for Thermostability by Temperature-Gradient Gel Electrophoresis
- 20. Inhibition of Proteinase K by Mercury
- V. Protein Engineering of Subtilisin Enzymes
- 21. Crystallographic Investigations of Subtilisin BPN' Mutants Engineered for Studying Thermal Stability
- 22. Structural and Functional Consequences of Engineering the High Alkaline Serine Protease PB92
- 23. Engineering Surface Charges in a Subtilisin
- 24. pH Dependence of the Catalytic Activity of a Subtilisin-like Proteinase
- 25. An Investigation of the Savinase Water Channel: Implications of Cavity Mutations
- 26. Substrate Specificity of Natural Variants and Genetically Engineered Intermediates of Bacillus lentus Alkaline Proteases
- 27. Unusual Ligand Binding at the Active Site Domain of an Engineered Mutant of Subtilisin BL
- 28. Studies on the Structure and Function of Subtilisin E by Protein Engineering
- 29. Structural Changes Leading to Increased Enzymatic Activity in an Engineered Variant of Bacillus lentus Subtilisin.