Scanning Electron Microscopy of Cerebellar Cortex /

Scanning Electron Microscopy of Cerebellar Cortex /

Detalles Bibliográficos
Autor principal: Castejón, Orlando. (Autor)
Autor Corporativo: SpringerLink (Online service)
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer US : Imprint: Springer, 2003.
Edición:1st ed. 2003.
Materias:
Neurosciences.
Microscopy.
Biological Microscopy.
Tabla de Contenidos:
  • 1. Sample Preparation Methods for Scanning Electron Microscopy
  • Conventional SEM technique or slicing technique
  • Fixation procedures
  • The prefixation state of the nerve tissue
  • Vascular perfusion fixation technique
  • Criteria for good fixation and optimal preservation of nerve tissue
  • Trimming procedure and obtaining nerve tissue slices
  • Dehydration
  • Critical point drying method
  • Specimen mounting and orientation
  • Metal deposition
  • Nerve cell specimens coated with thick gold-palladium films
  • Special SEM preparation techniques
  • Concluding remarks
  • 2. The Cerebellar White Matter
  • Brief history
  • The afferent and efferent fibers
  • Concluding remarks
  • 3. Granule Cells
  • Short history
  • The three-layered structure of cerebellar cortex
  • Outer surface of intact granule cells
  • Inner organization of fractured granule cells
  • Granule cell processes
  • Concluding remarks
  • 4. The Mossy Fiber Glomerulus
  • The long history
  • The mossy fiber-granule cell synaptic relationship
  • Concluding remarks
  • 5. Golgi Cells
  • Short history
  • Scanning electron microscopy of unfractured Golgi cells
  • Scanning electron microscopy of fractured Golgi cells
  • Concluding remarks
  • 6. Unipolar Brush Cells
  • Recent history and three-dimensional morphology
  • Future research
  • 7. Lugaro Cells
  • Short history
  • Three-dimensional morphology
  • Future research
  • 8. Purkinje Cells
  • The long history
  • Three-dimensional morphology and outer surface
  • Synaptic relationship with parallel fibers
  • Concluding remarks
  • 9. Climbing Fibers
  • Brief history
  • Intracortical course
  • Concluding remarks
  • 10. The Basket Cells
  • Brief history
  • Three-dimensional morphology
  • Concluding remarks
  • 11. Stellate Cells
  • Brief history
  • Three-dimensional morphology
  • Concluding remarks
  • 12. Cerebellar Glial Cells
  • Brief history
  • Oligodendrocytes
  • The velate protoplasmic astrocytes
  • Bergmann glial cells
  • Concluding remarks
  • 13. Cerebellar Capillaries
  • Short history
  • Three-dimensional morphology of cerebellar capillaries
  • Contribution of SEM to the cerebellar blood-brain barrier structure and function
  • Concluding remarks
  • 14. Contribution of Scanning Electron Microscopy to Cerebellar Neurobiology
  • The characterization of afferent and efferent fibers in the cerebellar white matter
  • Three-dimensional visualization of unfractured and fractured neurons
  • SEM as a high resolution tool for tracing cerebellar intracortical circuits
  • The three-dimensional morphology of synaptic connections
  • The three-dimensional morphology of glial cells
  • Contribution to the information processing in the cerebellar cortex
  • The three-dimensional design of cerebellar cortex
  • References.

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