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Signal Transduction Protocols /

Bibliographic Details
Main Authors: Kendall, David A. (Author), Hill, Stephen J. (Author)
Corporate Author: SpringerLink (Online service)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 1995.
Edition:1st ed. 1995.
Series:Methods in Molecular Biology, 41
Subjects:
Table of Contents:
  • The Problems and Pitfalls of Radioligand Binding
  • Solubilization and Purification of a Functional Ionotropic Excitatory Amino Acid Receptor
  • Autoradiography of ?1-and ?2-Adrenoceptors
  • In Situ Hybridization
  • Measurement of the GTPase Activity of Signal-Transducing G-Proteins in Neuronal Membranes
  • Measurement of Adenylyl Cyclase Activity in Cell Membranes
  • The Measurement of Cyclic AMP Levels in Biological Preparations
  • Mass Measurements of Cyclic AMP Formation by Radioimmunoassay, Enzyme Immunoassay, and Scintillation Proximity Assay
  • Measurement of Cyclic GMP Formation
  • Cyclic AMP-Dependent Protein Kinase Activity Ratio Assay
  • Purification and Activation of Cyclic GMP-Dependent Protein Kinase
  • The Analysis and Assay of Cyclic Nucleotide Phosphodiesterase Isoenzyme Activity
  • Separation of Labeled Inositol Phosphate Isomers by High-Pressure Liquid Chromatography (HPLC)
  • Mass Measurement of Key Phosphoinositide Cycle Intermediates
  • Measurement of Phospholipase C Activity in Brain Membranes
  • Measurement of Diacylglycerol
  • Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura-2
  • Measurement of Ca2+ Fluxes in Permeabilized Cells Using 45Ca2+ and Fluo-3
  • Single-Cell Calcium Imaging
  • Characterization of Calcium/Calmodulin-Stimulated Protein Kinase II
  • Protein Kinase C
  • The Measurement of Phospholipase D-Linked Signaling in Cells
  • Phospholipase A2 Activity
  • Techniques for the Measurement of Nitric Oxide.