Site-Specific Protein Labeling : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Gautier, Arnaud. (Editor ), Hinner, Marlon J. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2015.
Edición:1st ed. 2015.
Colección:Methods in Molecular Biology, 1266
Materias:
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245 1 0 |a Site-Specific Protein Labeling :  |b Methods and Protocols /  |c edited by Arnaud Gautier, Marlon J. Hinner. 
250 |a 1st ed. 2015. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2015. 
300 |a XII, 267 p. 72 illus., 34 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 1266 
505 0 |a How FlAsH got its Sparkle:Historical Recollections of the Biarsenical-tetracysteine Tag -- Site-Specific Protein Labeling in the Pharmaceutical Industry - Experiences from Novartis Drug Discovery -- Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins -- Considerations and Protocols for the Synthesis of Custom Protein Labeling Probes -- 2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine Residues -- Fluorescent Labeling for Patch Clamp Fluorometry (PCF) Measurements of Real-Time Protein Motion in Ion Channels -- Fluorescent Labeling Of SNAP-Tagged Proteins In Cells -- HaloTag Technology For Specific And Covalent Labeling Of Fusion Proteins -- Ligation Of Synthetic Peptides To Proteins Using Semisynthetic Protein Trans-Splicing -- Chemical-tag Labeling Of Proteins Using Fully Recombinant Split Inteins -- Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase Catalyzed Site-Specific Protein Labeling -- Site-Specific Biotinylation Of Purified Proteins Using BirA.- Site-specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist -- BONCAT - Metabolic Labeling, Click Chemistry, and Affinity Purification Of Newly Synthesized Proteomes -- Genetic Encoding Of Unnatural Amino Acids For Labeling Proteins -- Labeling Proteins By Affinity-Guided DMAP Chemistry -- Ligand-Directed Tosyl Chemistry For Selective Native Protein Labeling In Vitro, In Cells, and In Vivo. 
650 0 |a Proteins . 
650 1 4 |a Protein Science. 
650 2 4 |a Protein Structure. 
700 1 |a Gautier, Arnaud.  |e editor. 
700 1 |a Hinner, Marlon J.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks