Site-Specific Protein Labeling : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Gautier, Arnaud. (Editor ), Hinner, Marlon J. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2015.
Edición:1st ed. 2015.
Colección:Methods in Molecular Biology, 1266
Materias:
Tabla de Contenidos:
  • How FlAsH got its Sparkle:Historical Recollections of the Biarsenical-tetracysteine Tag
  • Site-Specific Protein Labeling in the Pharmaceutical Industry - Experiences from Novartis Drug Discovery
  • Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins
  • Considerations and Protocols for the Synthesis of Custom Protein Labeling Probes
  • 2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine Residues
  • Fluorescent Labeling for Patch Clamp Fluorometry (PCF) Measurements of Real-Time Protein Motion in Ion Channels
  • Fluorescent Labeling Of SNAP-Tagged Proteins In Cells
  • HaloTag Technology For Specific And Covalent Labeling Of Fusion Proteins
  • Ligation Of Synthetic Peptides To Proteins Using Semisynthetic Protein Trans-Splicing
  • Chemical-tag Labeling Of Proteins Using Fully Recombinant Split Inteins
  • Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase Catalyzed Site-Specific Protein Labeling
  • Site-Specific Biotinylation Of Purified Proteins Using BirA.- Site-specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist
  • BONCAT – Metabolic Labeling, Click Chemistry, and Affinity Purification Of Newly Synthesized Proteomes
  • Genetic Encoding Of Unnatural Amino Acids For Labeling Proteins
  • Labeling Proteins By Affinity-Guided DMAP Chemistry
  • Ligand-Directed Tosyl Chemistry For Selective Native Protein Labeling In Vitro, In Cells, and In Vivo.