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170227s2017 xxu| s |||| 0|eng d |
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|a 9781493968206
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| 024 |
7 |
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|a 10.1007/978-1-4939-6820-6
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Thermogenic Fat :
|b Methods and Protocols /
|c edited by Jun Wu.
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| 250 |
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|a 1st ed. 2017.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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| 300 |
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|a XI, 214 p. 42 illus., 30 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1566
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| 505 |
0 |
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|a Isolation, Primary Culture, and Differentiation of Preadipocytes from Mouse Brown Adipose Tissue -- Isolation of Mouse Stromal Vascular Cells for Monolayer Culture -- Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells -- Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes -- Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells -- Isolation of Immune Cells from Adipose Tissue -- Differentiation and Metabolic Interrogation of Human Adipocytes -- Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines -- Gene Expression and Histological Analysis of Activated Brown Adipocytes in Adipose Tissue -- Genetic Mouse Models: The Powerful Tools to Study Fat Tissues -- Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function -- Bioenergetic Analyses in Adipose Tissue -- Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry -- Isolation and Patch-Clamp of Primary Adipocytes -- In Vitro Approaches to Model and Study Communications Between Adipose Tissue and the Liver -- Identification and Quantification of Human Brown Adipose Tissue -- Designing 3D Adipospheres for Quantitative Metabolic Study -- Culture and Sampling of Primary Adipose Tissue in Practical Microfluidic Systems -- Using Thermogenic Beige Cells to Identify Biologically Active Small Molecules and Peptides.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Wu, Jun.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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