Schwann Cells : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Monje, Paula V. (Editor ), Kim, Haesun A. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:1st ed. 2018.
Colección:Methods in Molecular Biology, 1739
Materias:
Tabla de Contenidos:
  • Isolation of Schwann Cell Precursors from Rodents
  • Preparation of Neonatal Rat Schwann cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies
  • Isolation and Expansion of Schwann Cells from Transgenic Mouse Models
  • Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers
  • Detailed Protocols for the Isolation, Culture, Enrichment, and Immunostaining of Primary Human Schwann cells
  • Magnetic-activated Cell Sorting for the Fast and Efficient Separation of Human and Rodent Schwann Cells from Mixed Cell Populations
  • Culture and Expansion of Rodent and Porcine Schwann Cell for Pre-clinical Animal Studies
  • Chemical Conversion of Human Fibroblasts into functional Schwann Cells
  • Derivation of Fate-committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats
  • Human Induced Pluripotent Stem Cell (hiPS cell)-derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-derived Schwann Cells
  • Generation and Use of Merlin-Deficient Human Schwann Cells for a High-Throughput Chemical Genomics Screening Assay
  • Lentiviral Transduction of Rat Schwann Cells and Dorsal Root Ganglia Neurons for In Vitro Myelination Studies
  • Preservation, Sectioning and Staining of Schwann Cell Cultures for Transmission Electron Microscopy Analysis
  • Scalable Differentiation and Dedifferentiation Assays using Neuron-free Schwann Cell Cultures
  • The Pseudopod System for Axon-glia Interactions: Stimulation and Isolation of Schwann cell Protrusions that form in Response to Axonal Membranes
  • In vitro Analysis of the Role of Schwann Cells on Axonal Degeneration and Regeneration using Sensory Neurons from Dorsal Root Ganglia
  • A Culture Model To Study Neuron – Schwann cell – Astrocyte Interactions
  • Preparation of Matrices of Variable Stiffness for the Study of Mechanotransduction in Schwann Cell Development
  • Purification of Exosomes from Primary Schwann cells, RNA Extraction and Next Generation Sequencing of Exosomal RNAs
  • 3D Cancer Migration Assay with Schwann Cells
  • Teased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis
  • Whole Mount Immunostaining on Mouse Sciatic Nerves to Visualize Events of Peripheral Nerve Regeneration
  • The use of Low Vacuum Scanning Electron Microscopy (LVSEM) to Analyze Peripheral Nerve Myelin
  • Analysis of Myelinating Schwann Cells in Human Skin Biopsies
  • Whole Mount In Situ Hybridization and Immunohistochemistry for Myelin in Zebra Fish Larvae
  • Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve to Visualize Myelin
  • Live Imaging of Schwann Cell Development in Zebra Fish
  • Transplantation of Adult Rat Schwann Cells into the Injured Spinal Cord
  • Schwann Cell Transplantation Methods Using Biomaterials
  • Viral Transduction of Schwann Cells for Peripheral Nerve Repair
  • Intraspinal Delivery of Schwann Cells for Spinal Cord Injury. .