Type 2 Immunity : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Reinhardt, R. Lee. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:1st ed. 2018.
Colección:Methods in Molecular Biology, 1799
Materias:
Tabla de Contenidos:
  • A Fungal Protease Model To Interrogate Allergic Lung Immunity
  • Use of the Litomosoides sigmodontis Infection Model of Filariasis to study Type 2 Immunity
  • Production of Hymenolepis diminuta in the Laboratory: An Old Research Tool With New Clinical Applications
  • A Mouse Model of Peanut Allergy Induced By Sensitization through the Gastrointestinal Tract
  • Induction and Characterization of the Allergic Eye Disease Mouse Model
  • Isolation and Purification of Epithelial and Endothelial Cells from Mouse Lung
  • In vitro and In vivo IgE/Antigen Mediated Mast Cell Activation
  • The use of Human and Mouse Mast Cell and Basophil Cultures to Assess type-2 Inflammation
  • Isolation and Identification of Group 2 Innate Lymphoid Cells in Settings of Type 2 Inflammation
  • Determination of the Fate and Function of Innate Lymphoid Cells Following Adoptive Transfer of Innate Lymphoid Cell Precursors
  • Characterization of Thymic Development of Natural Killer T Cell Subsets by Multiparameter Flow Cytometry
  • Characterization of Mouse gd T Cell Subsets in the Setting of type-2 Immunity
  • The Identification of Allergen-derived T cell Epitopes
  • Generation of Allergen-specific Tetramers for a Murine Model of Airway Inflammation
  • The Generation and Use of Allergen-specific TCR Transgenic Animals
  • Using Cytokine Reporter Mice to Visualize Type-2 immunity in vivo
  • Live Imaging of IL-4 expressing T Follicular Helper Cells in Explanted Lymph Nodes
  • Imaging Precision Cut Lung Slices to Visualize Leukocyte Localization and Trafficking
  • Study of IgE-producing B cells using the Verigem Fluorescent Reporter Mouse
  • Chromatin Preparation from Murine Eosinophils for Genome-wide Analyses
  • A Sensitive and Integrated Approach to Profile Messenger RNA from Samples with Low Cell Numbers
  • An Integrated and Semi-automated Micro-scaled Approach to Profile Cis-Regulatory Elements by Histone Modification ChIP-Seq for Large-scale Epigenetic Studies
  • Library preparation for ATAC-sequencing of Mouse CD4+ T Cells Isolated from the Lung and Lymph Nodes after Helminth Infection
  • Identification of Functionally-relevant microRNAs in the Regulation of Allergic Inflammation
  • The Use of Biodegradable Nanoparticles for Tolerogenic Therapy of Allergic Inflammation
  • Assessing the Mouse Intestinal Microbiota in Settings of type-2 Immune Responses
  • The use of CRISPR-Cas9 Technology to Reveal Important Aspects of Human Airway Biology
  • A Consistent Method to Identify and Isolate Mononuclear Phagocytes from Human Lung and Lymph Nodes
  • Organoid Cultures for Assessing Intestinal Epithelial Differentiation and Function in Response to Type-2 Inflammation
  • Utilization of Air-liquid Interface Cultures as an in vitro Model to Assess Primary Airway Epithelial Cell Responses to the Type 2 Cytokine Interleukin 13.