Riboswitches : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Serganov, Alexander. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 540
Materias:
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020 |a 9781597455589 
024 7 |a 10.1007/978-1-59745-558-9  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Riboswitches :  |b Methods and Protocols /  |c edited by Alexander Serganov. 
250 |a 1st ed. 2009. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2009. 
300 |a XI, 366 p. 65 illus. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 540 
505 0 |a Predicting Riboswitch Regulation on a Genomic Scale -- Enzymatic Ligation Strategies for the Preparation of Purine Riboswitches with Site-Specific Chemical Modifications -- Application of Fluorescent Measurements for Characterization of Riboswitch–Ligand Interactions -- Transcriptional Approaches to Riboswitch Studies -- Kinetics of Riboswitch Regulation Studied By In Vitro Transcription -- Molecular Basis of RNA-Mediated Gene Regulation on the Adenine Riboswitch by Single-Molecule Approaches -- Methods for Analysis of Ligand-Induced RNA Conformational Changes -- Monitoring RNA–Ligand Interactions Using Isothermal Titration Calorimetry -- Preparation and Crystallization of Riboswitch–Ligand Complexes -- Crystallization of the glmS Ribozyme-Riboswitch -- Riboswitch Conformations Revealed by Small-Angle X-Ray Scattering -- Time-Resolved NMR Spectroscopy: Ligand-Induced Refolding of Riboswitches -- Analysis of the RNA Backbone: Structural Analysis of Riboswitches by In-Line Probing and Selective 2’-Hydroxyl Acylation and Primer Extension -- Identification of Metabolite–Riboswitch Interactions Using Nucleotide Analog Interference Mapping and Suppression -- RNA-Dependent RNA Switches in Bacteria -- Probing mRNA Structure and sRNA–mRNA Interactions in Bacteria Using Enzymes and Lead(II) -- Structural Probing of RNA Thermosensors -- Ribosomal Initiation Complexes Probed by Toeprinting and Effect of trans-Acting Translational Regulators in Bacteria -- Isolation and Characterization of the Heat Shock RNA 1 -- Analysis of tRNA-Directed Transcription Antitermination in the T Box System In Vivo -- In Vitro Selection of Conformational Probes for Riboswitches -- A Green Fluorescent Protein (GFP)-Based Plasmid System to Study Post-Transcriptional Control of Gene Expression In Vivo -- High-Throughput Screens to Discover Synthetic Riboswitches -- A Mammalian Cell-Based Assay for Screening Inhibitors of RNA Cleavage -- In Vitro Selection of glmS Ribozymes. 
650 0 |a Biochemistry. 
650 0 |a Nucleic acids. 
650 0 |a Gene expression. 
650 1 4 |a Biochemistry, general. 
650 2 4 |a Nucleic Acid Chemistry. 
650 2 4 |a Gene Expression. 
700 1 |a Serganov, Alexander.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks