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110414s2009 xxu| s |||| 0|eng d |
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|a 9781597455589
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024 |
7 |
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|a 10.1007/978-1-59745-558-9
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
1 |
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|a Riboswitches :
|b Methods and Protocols /
|c edited by Alexander Serganov.
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250 |
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|a 1st ed. 2009.
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260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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300 |
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|a XI, 366 p. 65 illus. :
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 540
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|a Predicting Riboswitch Regulation on a Genomic Scale -- Enzymatic Ligation Strategies for the Preparation of Purine Riboswitches with Site-Specific Chemical Modifications -- Application of Fluorescent Measurements for Characterization of Riboswitch–Ligand Interactions -- Transcriptional Approaches to Riboswitch Studies -- Kinetics of Riboswitch Regulation Studied By In Vitro Transcription -- Molecular Basis of RNA-Mediated Gene Regulation on the Adenine Riboswitch by Single-Molecule Approaches -- Methods for Analysis of Ligand-Induced RNA Conformational Changes -- Monitoring RNA–Ligand Interactions Using Isothermal Titration Calorimetry -- Preparation and Crystallization of Riboswitch–Ligand Complexes -- Crystallization of the glmS Ribozyme-Riboswitch -- Riboswitch Conformations Revealed by Small-Angle X-Ray Scattering -- Time-Resolved NMR Spectroscopy: Ligand-Induced Refolding of Riboswitches -- Analysis of the RNA Backbone: Structural Analysis of Riboswitches by In-Line Probing and Selective 2’-Hydroxyl Acylation and Primer Extension -- Identification of Metabolite–Riboswitch Interactions Using Nucleotide Analog Interference Mapping and Suppression -- RNA-Dependent RNA Switches in Bacteria -- Probing mRNA Structure and sRNA–mRNA Interactions in Bacteria Using Enzymes and Lead(II) -- Structural Probing of RNA Thermosensors -- Ribosomal Initiation Complexes Probed by Toeprinting and Effect of trans-Acting Translational Regulators in Bacteria -- Isolation and Characterization of the Heat Shock RNA 1 -- Analysis of tRNA-Directed Transcription Antitermination in the T Box System In Vivo -- In Vitro Selection of Conformational Probes for Riboswitches -- A Green Fluorescent Protein (GFP)-Based Plasmid System to Study Post-Transcriptional Control of Gene Expression In Vivo -- High-Throughput Screens to Discover Synthetic Riboswitches -- A Mammalian Cell-Based Assay for Screening Inhibitors of RNA Cleavage -- In Vitro Selection of glmS Ribozymes.
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650 |
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|a Biochemistry.
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650 |
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|a Nucleic acids.
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650 |
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|a Gene expression.
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650 |
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4 |
|a Biochemistry, general.
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650 |
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4 |
|a Nucleic Acid Chemistry.
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650 |
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|a Gene Expression.
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|a Serganov, Alexander.
|e editor.
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710 |
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|a SpringerLink (Online service)
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773 |
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|t Springer eBooks
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