Riboswitches : Methods and Protocols /
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2009.
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| Edición: | 1st ed. 2009. |
| Colección: | Methods in Molecular Biology,
540 |
| Materias: |
Tabla de Contenidos:
- Predicting Riboswitch Regulation on a Genomic Scale
- Enzymatic Ligation Strategies for the Preparation of Purine Riboswitches with Site-Specific Chemical Modifications
- Application of Fluorescent Measurements for Characterization of Riboswitch–Ligand Interactions
- Transcriptional Approaches to Riboswitch Studies
- Kinetics of Riboswitch Regulation Studied By In Vitro Transcription
- Molecular Basis of RNA-Mediated Gene Regulation on the Adenine Riboswitch by Single-Molecule Approaches
- Methods for Analysis of Ligand-Induced RNA Conformational Changes
- Monitoring RNA–Ligand Interactions Using Isothermal Titration Calorimetry
- Preparation and Crystallization of Riboswitch–Ligand Complexes
- Crystallization of the glmS Ribozyme-Riboswitch
- Riboswitch Conformations Revealed by Small-Angle X-Ray Scattering
- Time-Resolved NMR Spectroscopy: Ligand-Induced Refolding of Riboswitches
- Analysis of the RNA Backbone: Structural Analysis of Riboswitches by In-Line Probing and Selective 2’-Hydroxyl Acylation and Primer Extension
- Identification of Metabolite–Riboswitch Interactions Using Nucleotide Analog Interference Mapping and Suppression
- RNA-Dependent RNA Switches in Bacteria
- Probing mRNA Structure and sRNA–mRNA Interactions in Bacteria Using Enzymes and Lead(II)
- Structural Probing of RNA Thermosensors
- Ribosomal Initiation Complexes Probed by Toeprinting and Effect of trans-Acting Translational Regulators in Bacteria
- Isolation and Characterization of the Heat Shock RNA 1
- Analysis of tRNA-Directed Transcription Antitermination in the T Box System In Vivo
- In Vitro Selection of Conformational Probes for Riboswitches
- A Green Fluorescent Protein (GFP)-Based Plasmid System to Study Post-Transcriptional Control of Gene Expression In Vivo
- High-Throughput Screens to Discover Synthetic Riboswitches
- A Mammalian Cell-Based Assay for Screening Inhibitors of RNA Cleavage
- In Vitro Selection of glmS Ribozymes.