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120213s2012 xxu| s |||| 0|eng d |
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|a 9781617794742
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|a 10.1007/978-1-61779-474-2
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Ubiquitin Family Modifiers and the Proteasome :
|b Reviews and Protocols /
|c edited by R. Jürgen Dohmen, Martin Scheffner.
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|a 1st ed. 2012.
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2012.
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|a XVI, 666 p. 133 illus., 39 illus. in color. :
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 832
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|a Three Decades of Studies to Understand the Functions of the Ubiquitin Family -- Structure and Evolution of Ubiquitin and Ubiquitin-Related Domains -- Deciphering Tissue-Specific Ubiquitylation by Mass Spectrometry -- Analysis of Cellular SUMO and SUMO-Ubiquitin Hybrid Conjugates -- Recombinant Reconstitution of Sumoylation Reactions In vitro -- Reconstitution of SUMO-Dependent Ubiquitylation In vitro -- Detection and Analysis of FAT10 Modification -- Isolation of NEDDylated Proteins in Human Cells -- The ISG15 Conjugation System -- Pupylation: Proteasomal Targeting by a Protein Modifier in Bacteria -- Role of UbF Family Modifiers and their Binding Proteins in Cell Signaling -- Isolation of Ubiquitylated Proteins using Tandem Ubiquitin-Binding Entities (TUBEs) -- Using Linkage-Specific Monoclonal Antibodies to Analyze Cellular Ubiquitination -- Measuring Rates of Ubiquitin Chain Formation as a Functional Readout of Ligase Activity -- Synthesis and Analysis of K11-Linked Ubiquitin Chains -- Synthesis and Analysis of Linear Ubiquitin Chains -- Detection and Quantitation of SUMO Chains by Mass Spectrometry -- Bioinformatical Detection of Recognition Factors for Ubiquitin and SUMO -- Surface Plasmon Resonance to Measure Interactions of UbFs with Their Binding Partners -- Identifying and Studying Ubiquitin Receptors by NMR -- Exploring the Role of p97 and its UBX-Domain Cofactors Through Identification of Their Interacting Proteins -- Assembly and Function of the Proteasome -- Using Native Gel Electrophoresis and Phosphofluoroimaging to Analyse GFP-tagged Proteasomes -- Disulfide Engineering to Map Subunit Interactions in the Proteasome and Other Macromolecular Complexes -- Using DNA Damage Sensitivity Phenotypes to Characterize Mutations Affecting Proteasome Function -- Analysing Properties of Proteasome Inhibitors using Kkinetic and X-Ray Crystallographic Studies -- Immunoproteasome-Specific Inhibitors and Their Application -- Binding of Ubiquitin Conjugates to Proteasomes as Visualized with Native Gels -- Affinity Purification of Mmammalian 26S Proteasomes Using an Ubiquitin-Like Domain -- Using siRNA Techniques to Dissect Proteasome Assembly Pathways in Mammalian Cells -- Reconstitution of PA700, the 19S Regulatory Particle, from Purified Precursor Complexes -- Cellular Responses to Misfolded Proteins and Protein Aggregates -- Live-Cell Imaging of Ubiquitin-Proteasome System Function -- Analysis of Chaperone-assisted Ubiquitylation -- Use of CPY* and its Derivatives to Study Protein Quality Control in Various Cell Compartments -- Assays to Measure ER-Associated Degradation in Yeast -- SDS-PAGE Techniques to Study Ubiquitin-Like Conjugation Systems in Yeast Autophagy -- Analysis of Ubiquitin-Dependent Proteolysis in C. elegans -- Structural Insights into Functional Modes of Proteins Involved in Ubiquitin Family Pathways -- Identification and Application of NEDD8 E1 Inhibitors -- Formation of Ubiquitin Dimers via Azide Alkyne Click Reaction -- Synthesis of Atypical Diubiquitin Chains -- TIPI - TEV Protease-Mediated Induction of Protein Instability -- PROTAC-Induced Proteolytic Targeting.
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|a Proteins .
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|a Proteomics.
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|a Protein Science.
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|a Proteomics.
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|a Dohmen, R. Jürgen.
|e editor.
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|a Scheffner, Martin.
|e editor.
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|a SpringerLink (Online service)
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|t Springer eBooks
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