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02708nam a22003615i 4500 |
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20211005170202.0 |
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cr nn 008mamaa |
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160620s2016 gw | s |||| 0|eng d |
| 020 |
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|a 9783319266664
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| 024 |
7 |
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|a 10.1007/978-3-319-26666-4
|2 doi
|
| 040 |
|
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a Tumor Microenvironment :
|b Study Protocols /
|c edited by Constantinos Koumenis, Lisa M. Coussens, Amato Giaccia, Ester Hammond.
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| 250 |
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|a 1st ed. 2016.
|
| 260 |
# |
# |
|a Cham :
|b Springer International Publishing :
|b Imprint: Springer,
|c 2016.
|
| 300 |
|
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|a VIII, 297 p. 85 illus., 64 illus. in color. :
|b online resource.
|
| 336 |
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|a text
|b txt
|2 rdacontent
|
| 337 |
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|a computer
|b c
|2 rdamedia
|
| 338 |
|
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|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Advances in Experimental Medicine and Biology,
|v 899
|
| 505 |
0 |
|
|a 1.Staining against phospho-H2AX (γ-H2AX) as a marker for DNA damage and genomic instability in cancer tissues and cells -- 2.Measuring DNA replication rates in hypoxic conditions -- 3.Isolation of Proteins on Nascent DNA in hypoxia and reoxygenation conditions -- 4.In vivo interrogation of the hypoxic transcriptome of solid tumors: optimizing hypoxic probe labelling with Laser-Capture Microdissection for isolation of high-quality RNA for deep sequencing analysis -- 5.Gas chromatography coupled to mass spectrometry (GC-MS) to study metabolism in cultured cells -- 6.Probing Cancer Cell Metabolism using NMR Spectroscopy -- 7.Measuring the Impact of Microenvironmental Conditions on Mitochondrial Dehydrogenases Activity in Cultured Cells -- 8.Measuring Autophagy in the Context of Cancer -- 9.Methods for studying autophagy within the tumor microenvironment -- 10.Using 3 Dimensional Culture (Spheroids) as an in vitro Model of Tumour Hypoxia -- 11.Isolation of Glioma Initiating Cells for Biological Study -- 12.Isolation of mouse and human tumor associated macrophages -- 13.Mouse models of brain metastasis for unravelling tumour progression -- 14.Quantification of lung metastases from in vivo mouse models -- 15.A multimodal data analysis approach for targeted drug discovery involving topological data analysis (TDA) -- 16.Efficient protocol for the identification of hypoxic cell radiosensitisers.
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| 650 |
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0 |
|a Cancer research.
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| 650 |
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0 |
|a Molecular biology.
|
| 650 |
|
0 |
|a Immunology.
|
| 650 |
1 |
4 |
|a Cancer Research.
|
| 650 |
2 |
4 |
|a Molecular Medicine.
|
| 650 |
2 |
4 |
|a Immunology.
|
| 700 |
1 |
|
|a Koumenis, Constantinos.
|e editor.
|
| 700 |
1 |
|
|a Coussens, Lisa M.
|e editor.
|
| 700 |
1 |
|
|a Giaccia, Amato.
|e editor.
|
| 700 |
1 |
|
|a Hammond, Ester.
|e editor.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|